Abstract
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Atomic Structure of PDE4: Insights into Phosphodiesterase Mechanism and Specificity
Robert X. Xu, Anne M. Hassell, Dana Vanderwall, Millard H. Lambert, William D. Holmes, Michael A. Luther, Warren J. Rocque, Michael V. Milburn, Yingdong Zhao, Hengming Ke, and Robert T. Nolte

Supplementary Material

Supplemental Figure 1. Sequence alignment and secondary structure assignment of representative members from the 10 PDE gene families. Residues are colored by property, with acidic residues red, basic residues blue, and hydrophobic residues green. Residues that are conserved across all 10 PDE gene families are highlighted with yellow boxes. Annotations below each column of residues denote function/significance as follows: 1, binds to ME1; 2, binds to ME2; W, interacts with metal bound waters; A, other active-site residues. Long inserts between H6 and H7 in PDE3, and between H15 and H16 in PDE1 and PDE3, were deleted, as identified here with red underlining.


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