A jawbone was found by a Chinese monk in a holy cave high on the Tibetan Plateau. 

DONGJU ZHANG/LANZHOU UNIVERSITY

First fossil jaw of Denisovans finally puts a face on elusive human relatives

Thirty-nine years ago, a Buddhist monk meditating in a cave on the edge of the Tibetan Plateau found something strange: a human jawbone with giant molars. The fossil eventually found its way to scientists. Now, almost 4 decades later, a groundbreaking new way to identify human fossils based on ancient proteins shows the jaw belonged to a Denisovan, a mysterious extinct cousin of Neanderthals.

The jawbone is the first known fossil of a Denisovan outside of Siberia's Denisova Cave in Russia, and gives paleoanthropologists their first real look at the face of this lost member of the human family. "We are finally ‘cornering’ the elusive Denisovans," paleoanthropologist María Martinón-Torres of the National Research Center on Human Evolution in Burgos, Spain, wrote in an email. "We are getting their smiles!"

Together, the jaw's anatomy and the new method of analyzing ancient proteins could help researchers learn whether other mysterious fossils in Asia are Denisovan. "We now can use this fossil and this wonderful new tool to classify other fossil remains that we can't agree on," says paleoanthropologist Aida Gomez-Robles of University College London, who reviewed the paper, which appears in Nature this week.

The international team of researchers also reports that the jawbone is at least 160,000 years old. Its discovery pushes back the earliest known presence of humans at high altitude by about 120,000 years.

A massive search for Denisovans has been underway ever since paleogeneticists extracted DNA from the pinkie of a girl who lived more than 50,000 years ago in Denisova Cave and found she was a new kind of human. Max Planck Society researchers have since sequenced DNA from several Denisovans from the cave, but the fossils—isolated teeth and bits of bone—were too scanty to show what this enigmatic hominin looked like. Denisovans must have been widespread, because many living people in Melanesia and Southeast Asia carry traces of DNA from multiple encounters between modern humans and Denisovans. But although intriguing fossils across Asia could be Denisovan, they have not yielded the DNA that could confirm their identity.

Enter the new jawbone, found by an unidentified monk in Baishiya Karst Cave in Xiahe county in China at an altitude of 3200 meters on the margins of the Tibetan Plateau, according to co-author Dongju Zhang, an archaeologist at Lanzhou University in northwestern China. She traced the jawbone's discovery by interviewing local people in Xiahe, who told her they remembered human bones from the large cave, which is next to a Buddhist shrine and is still a holy place as well as a tourist attraction. Recognizing the jaw's unusual nature, the monk gave it to the sixth Gung-Thang living Buddha, one of China's officially designated "living Buddhas," who consulted scholars and then gave the jaw to Lanzhou University. The jawbone was so "weird" that researchers there didn't know how to classify it, and it sat on shelves for years, Zhang says.

The proteins in this lower jawbone identify it as Denisovan.

DONGJU ZHANG/LANZHOU UNIVERSITY

She and geologist Fahu Chen, also from Lanzhou University and the Institute of Tibetan Plateau Research in Beijing, showed the jaw to paleoanthropologist Jean-Jacques Hublin of the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany. After seeing its large molars—as big as ones found in Denisova Cave—Hublin immediately suspected it was Denisovan.

Max Planck paleogeneticists couldn't get DNA from the jaw, but Hublin's graduate student Frido Welker had found in his doctoral work that Neanderthals, modern humans, and Denisovans differ in the amino acid sequence of key proteins. Welker, now a postdoc at the University of Copenhagen, was able to extract collagen, a common structural protein, from a molar of the Xiahe jawbone. He found its amino acid sequence most closely matched that of Denisovans.

Other team members dated a carbonate crust that had formed on the skull by measuring the radioactive decay of uranium in the carbonate. They got a date of 160,000 years ago—a "firm minimum date" for the skull, says geochronologist Rainer Grün of Griffith University in Nathan, Australia, who is not a member of the team.

The date suggests Denisovans would have had tens of thousands of years to adapt to the altitude of Tibet by the time modern humans arrived in the region, some 30,000 to 40,000 years ago. Encounters between modern humans and Denisovans adapted to high altitude could explain how the Tibetans of today came by a Denisovan gene that helps them cope with thin air. "It seems likely that ancestral Tibetans interacted with Denisovans, as they began to move upslope," archaeologist David Madsen of the University of Texas in Austin wrote in an email.

The jaw's features could be a template for spotting other Denisovans. "Its distinct large molars and premolar roots differ from those of Neanderthals," and the jawbone "is very primitive and robust," says Hublin, who sees a resemblance to a jawbone found off the coast of Taiwan known as the Penghu mandible.

What anatomy can't confirm, proteins might. "The protein analyses allow us to see landscapes where DNA cannot reach"—from warmer climates or much more ancient sites where fragile DNA doesn't persist, Martinón-Torres says. Other researchers have a half-dozen fossils they want to test for proteins or compare with the Xiahe jaw.

The implications are far-reaching. "Forget the textbooks," says archaeologist Robin Dennell of the University of Sheffield in the United Kingdom. "Human evolution in Asia is far more complex than we currently understand, and probably does involve multiple lineages, some of which probably engaged with our species."

Meanwhile, Chen and Zhang did their first excavation at the cave in December 2018, with permission from local villagers and Buddhists. They dug two small trenches where they have already found stone tools and cut-marked rhino and other animal bones. "We do have hope we'll find more Denisovans," Zhang says.