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Temporal and spatial imaging of the proteome in living cells

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Temporal and spatial imaging of the proteome in living cells

06 November 2019

12:00 p.m. ET

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Cellular processes are orchestrated by many biomolecules operating in a spatially and temporally coordinated manner within a tiny volume. To uncover the organizational principles underlying these processes and their functional relevance using microscopy visualization, we have developed a fragment-tagging approach that fluorescently labels target proteins using our engineered split-fluorescent proteins. This approach has enabled systematic generation of knockin cell lines with endogenously labeled proteins through CRISPR/Cas9-mediated gene editing. We have also developed the epi-illumination selective plane illumination microscopy (eSPIM) light-sheet microscopy platform, enabling high-resolution, high-throughput imaging of multiple cell lines. Combining these two techniques has paved the way for systematic mapping of the spatial localization and temporal dynamics of proteins in living cells. In the closing section of the webinar, we will focus on electron-multiplying charge-coupled device (EMCCD) imaging technology involved in proteome research. Protein-driven phenomena tend to happen at very small scales of space and time. These challenging environments call for advanced light manipulation and detection technologies optimized for low-light conditions. We will discuss the outlook for future camera-based imaging for applications aiming to uncover gene position, activity, and the resultant protein’s role and fate.

During this webinar, the speakers will:

  • Explain split-fluorescent protein tags for efficient labeling of endogenous proteins in cell lines
  • Describe the eSPIM system for high-throughput volumetric imaging of living cells
  • Discuss the perspective of library-based fluorescence microscopy analysis as a primary discovery tool
  • Highlight selected technical aspects of imaging-camera performance relevant for efficient visualization of gene expression as part of the proteome analysis of living cells
  • Answer viewer questions live during the broadcast.

This webinar will last for approximately 60 minutes.

Speaker bios

Bo Huang, Ph.D.

University of California, San Francisco
San Francisco, CA

Dr. Huang is a professor of pharmaceutical chemistry and of biochemistry and biophysics (joint) at the University of California, San Francisco (UCSF), and a Chan Zuckerberg Biohub Investigator. He received his B.S. in chemistry from Peking University in 2001, and his Ph.D. in chemistry from Stanford University in 2006. After finishing postdoc work at Harvard University in 2009, he joined UCSF as an assistant professor, where he was promoted to associate professor in 2014 and professor in 2017. His research encompasses the areas of optical microscopy, bioengineering, biophysics, and cell biology. Dr. Huang has received many awards, including the GE and Science Prize for Young Life Scientists, the Searle Scholarship, the Packard Fellowship for Science and Engineering, the NIH Director’s New Innovator Award, the American Society for Cell Biology Early Career Life Scientist Award, and the UCSF Byers Award in Basic Science.

Marcin Barszczewski, Ph.D.

Andor Technology
Belfast, Northern Ireland

Dr. Barszczewski is an applications scientist and training specialist at Andor Technology. He has 15 years’ expertise in a wide range of imaging applications for CCD, EMCCD, and CMOS cameras. Barszczewski obtained his Ph.D. in neuroscience from the Max Planck Institute for Biophysical Chemistry and the Georg-August University in Göttingen, Germany. He has held various technical roles at Andor (now an Oxford Instruments company), advising customers in the fields of astronomy and low-light biomedical imaging as well as applications in the physical sciences, including X-ray and quantum imaging.

Jackie Oberst, Ph.D.

Washington, D.C.

Dr. Oberst did her undergraduate training at the University of Maryland, College Park, and her Ph.D. in Tumor Biology at Georgetown University, Washington D.C. She combined her interests in science and writing by pursuing an M.A. in Journalism from the Philip Merrill College of Journalism at the University of Maryland, College Park. Dr. Oberst joined Science/AAAS in 2016 as the Assistant Editor for Custom Publishing. Before then she worked at Nature magazine, the Howard Hughes Medical Institute, The Endocrine Society, and the National Institutes of Mental Health.

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