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Techniques for RNA Detection: Where Are We Headed?

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Techniques for RNA Detection: Where Are We Headed?

Recorded 27 August 2014

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As the messenger of genetic information, RNA plays an important regulatory role in cell and tissue development as well as during disease progression. Understanding the best ways to work with RNA and the various RNA detection methods can help scientists advance our understanding of gene expression patterns and elucidate the roles of different genomic elements in cellular function and dysfunction. A variety of RNA detection methods—including Northern blotting, in situ hybridization, and reverse transcription polymerase chain reaction—are already available; however, each comes with its own advantages and limitations in signal sensitivity, specificity, and stability. Additionally, most often detection is carried out on lysed cell samples, so a significant amount of information is lost. This webinar will bring together two experts who will share their knowledge and expertise in a variety of RNA detection methodologies, including one that utilizes live cells.

During the webinar the panelists will:

  • Discuss different RNA detection methods
  • Highlight the unique challenges and benefits of detecting RNA in live cells
  • Present some of their current research using RNA detection techniques
  • Answer audience questions during the live webinar!

To learn more about products or technologies related to this webinar, go to: www.emdmillipore.com/smartflare

Speaker bios

Martin J. Stoddart, Ph.D.

AO Research Institute Davos
Davos, Switzerland

Dr. Stoddart completed a Bachelor’s degree in biology at the University of Aberystwyth prior to earning a Master’s of Philosophy in cartilage biology from the AO Research Institute (ARI) Davos. He then carried out his doctoral research on cancer angiogenesis at the University of Nottingham, followed by an appointment in the Laboratory for Experimental Cartilage Research, initially as postdoc and then as a group head. During that time, he took a six-month sabbatical at the Center for Molecular Orthopaedics, Harvard Medical School/Brigham and Women’s Hospital, in Boston to learn viral gene transfer techniques. In 2005, he returned to ARI where he became a principal investigator in 2009.

Dr. Stoddart’s main research focus is using autologous stem cells and gene transfer to repair musculoskeletal tissues using a cell therapy approach. To this aim, he investigates novel cell identification and isolation methods. His research interests include the mechano-regulation of stem cell fate, in particular chondrogenic differentiation. He has authored over 45 scientific papers and seven book chapters and is the editor of the Mammalian Cell Viability Methods: Methods in Molecular Biology, Vol.740. He is an editor for Tissue Engineering Journal Parts A, B, C and BioMed Research International Orthopedics as well as a scientific editor for eCM Journal and an organizer for their yearly conference. He is a member of the Basic Science Education Committee for the Orthopaedic Research Society and an associate faculty member of the Faculty of 1000 Medicine.

In this webinar, Dr. Stoddart will show identifying cell fate of mesenchymal stem cells is more accurate and specific when studying the expression ratios of multiple targets as opposed to studying them in isolation. Monitoring these expression ratios in live cells would provide prospective gene expression data as opposed to traditional retrospective characterization.

Don Weldon

EMD Millipore
Temecula, CA

Mr. Weldon holds a Bachelor’s degree in cellular and molecular biology from San Diego State University. He also received a certificate in recombinant DNA technology from the same institution. He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers. His current focus involves working on a novel technology for RNA detection in live cells.

In this webinar, Mr. Weldon will cover the architecture and technology background for SmartFlare RNA detection probes, which enable the detection of specific RNA’s within live cells.

Tianna Hicklin, Ph.D.

Science/AAAS
Washington, DC

Dr. Hicklin studied biology at Colorado State University for her undergraduate education before earning a Ph.D. in neuroscience from the University of Colorado Anschutz Medical Campus. Prior to joining Science/AAAS, she worked as a science writer intern for the University of Colorado’s Office of Media and Public Relations in Denver, Colorado and for Brookhaven National Laboratory’s Media and Communications Office in Upton, New York. Dr. Hicklin is currently the assistant editor for the Science/AAAS Custom Publishing Office.

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