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Supersizing superresolution microscopy: Open-source approaches to accelerate your microscopy research

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Supersizing superresolution microscopy: Open-source approaches to accelerate your microscopy research

Recorded 24 October 2018


Three high-performance, open-source approaches—NanoJ-SRRF, NanoJ-SQUIRREL, and NanoJ-Fluidics—have recently been developed to enable and enhance optical superresolution microscopy in most modern microscopes. NanoJ-superresolution radical fluctuations (SRRF) is a new superresolution method enabling live-cell nanoscopy with illumination intensities orders of magnitude lower than techniques such as single-molecule localization microscopy (SMLM) or stimulated emission depletion (STED) microscopy can deliver. SRRF’s low phototoxicity allows unprecedented imaging for long acquisition times at resolutions equivalent to or better than those possible with structured illumination microscopy (SIM). NanoJ-SQUIRREL (superresolution quantitative image rating and reporting of error locations), an analytical approach that provides quantitative assessment of superresolution image quality, can guide researchers in optimizing imaging parameters. By comparing diffraction-limited images and superresolution equivalents of the same acquisition volume, this method generates a quality score and quantitative map of superresolution defects. NanoJ-Fluidics is a novel fluidics technique for automating complex sequences of treatment, labeling, and imaging of live and fixed cells with high reproducibility.

During the webinar, speakers will:

  • Showcase how SRRF benefits from spinning-disk acquisition and performs with EM-CCDs and sCMOS cameras
  • Demonstrate how NanoJ-Fluidics can be used to examine actin dynamics, nanoscale cell topography, and the role of adhesion contacts in mitosis
  • Answer your questions during the live broadcast!

This webinar will last for approximately 60 minutes

Speaker bios

Ricardo Henriques, Ph.D.

University College London and the Francis Crick Institute
London, United Kingdom

Dr. Henriques leads the Quantitative Imaging and NanoBioPhysics research group at University College London, with a satellite research laboratory at the Francis Crick Institute. A physicist by training, he earned his Ph.D. in computational and optical biophysics at Instituto de Medicina Molecular (Portugal), Institute Pasteur (France), and the Council for Scientific and Industrial Research (South Africa). During this time, he developed the QuickPALM algorithm, one of the most used and cited analytical methods in single-molecule localization microscopy. He briefly held a postdoctoral position at Institute Pasteur, studying HIV-1 infection and T-cell signaling through superresolution microscopy, before starting his own research group in late 2013. Dr. Henriques’ laboratory focuses on developing new analytical, optical, and biochemical methods for superresolution microscopy and using them to study viral host–pathogen interactions.

Geraint Wilde, Ph.D.

Andor Technology
Belfast, United Kingdom

Dr. Wilde attained a Ph.D. in Neuroscience in 1997 from the University of Southampton, UK, after which he continued in his interest in neuroscience with a postdoctoral position at the University of Warwick, UK. Having developed an interest in microscopy, he moved to the University of Liverpool, UK, to work in the laboratory of Michael White, focusing on intercellular signaling and gene expression through live-cell imaging. Dr. Wilde eventually pursued a commercial career in microscopy, focusing on a few different imaging solutions with different companies. He joined Andor Technology in 2009, where he is now product manager for microscopy solutions.

Jackie Oberst, Ph.D.

Washington, DC

Dr. Oberst did her undergraduate training at the University of Maryland, College Park, and her Ph.D. in Tumor Biology at Georgetown University, Washington D.C. She combined her interests in science and writing by pursuing an M.A. in Journalism from the Philip Merrill College of Journalism at the University of Maryland, College Park. Dr. Oberst joined Science/AAAS in 2016 as the Assistant Editor for Custom Publishing. Before then she worked at Nature magazine, the Howard Hughes Medical Institute, The Endocrine Society, and the National Institutes of Mental Health.

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