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The power of RNA: Broad application of RNA-based sequencing for transcriptome and genome analysis

This webinar is brought to you by the Science/AAAS Custom Publishing Office

The power of RNA: Broad application of RNA-based sequencing for transcriptome and genome analysis

Recorded 04 September 2018


Over the past decade, RNA-based sequencing has become the standard method for analyzing differential messenger RNA (mRNA) expression and for detecting both novel transcripts and allele-specific expression, as well as for identifying pre-mRNA intron–exon splice junctions. This versatile technique has been used extensively to analyze the whole transcriptome and is frequently combined with mRNA capture or ribosomal RNA depletion to enable more detailed analysis across the broad range of expression levels. In some studies, novel RNA-based approaches provide research answers that cannot be revealed by conventional DNA-sequencing approaches. Join our experts as they discuss how they have used RNA sequencing in different ways to solve research problems for which DNA sequencing alone in some cases proved insufficient.

During the webinar, the speakers will:

  • Introduce viewers to diverse approaches and tools for RNA-based sequencing analysis
  • Describe the utility of RNASeq data in characterizing tumors, including variants identified by RNASeq that conventional DNA sequencing failed to detect
  • Highlight the Expressed Exome Capture Sequencing (EecSeq) method for using an RNA-based approach to analyze species without an annotated genome
  • Answer your questions during the live broadcast!

This webinar will last for approximately 60 minutes.

Speaker bios

Jon Puritz, Ph.D.

University of Rhode Island
Kingston, RI

Since 2017, Dr. Puritz has been an assistant professor in the Department of Biological Sciences at the University of Rhode Island in Kingston. Previously, he graduated with a B.S. in biology from Brown University in Providence, Rhode Island, and in 2011 earned his Ph.D. in zoology from the University of Hawai‘i at Mānoa, specializing in ecology, evolution, and conservation biology. He completed postdoctoral training at Texas A&M–Corpus Christi and the Marine Science Center of Northeastern University, where he developed the Expressed Exome Capture Sequencing (EecSeq) method in collaboration with Dr. Kathleen Lotterhos. Dr. Puritz’s research interests include understanding how natural and anthropogenic processes impact the evolution of marine populations, and the development of genomics-based and bioinformatic methods for nonmodel organisms.

Heather Geiger, B.A.

New York Genome Center
New York, NY

Ms. Geiger is a senior bioinformatics analyst at the New York Genome Center, focusing on RNASeq. She previously worked with Chris Mason at Weill Cornell Medicine, researching gene expression in the axons of developing neurons. At the New York Genome Center, she undertakes RNASeq projects related to neurodevelopment and neurodegenerative disease, cancer, and autoimmune disease. She has also done extensive work on benchmarking best practices for RNASeq analysis and developed an automated pipeline to analyze RNASeq data. She has performed the analysis on several single-cell RNA projects, including those conducted on plate-based and 10x Genomics platforms.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently, Dr. Sanders is the Director and Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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