Over the past decade, RNA-based sequencing has become the standard method for analyzing differential messenger RNA (mRNA) expression and for detecting both novel transcripts and allele-specific expression, as well as for identifying pre-mRNA intron–exon splice junctions. This versatile technique has been used extensively to analyze the whole transcriptome and is frequently combined with mRNA capture or ribosomal RNA depletion to enable more detailed analysis across the broad range of expression levels. In some studies, novel RNA-based approaches provide research answers that cannot be revealed by conventional DNA-sequencing approaches. Join our experts as they discuss how they have used RNA sequencing in different ways to solve research problems for which DNA sequencing alone in some cases proved insufficient.
During the webinar, the speakers will:
- Introduce viewers to diverse approaches and tools for RNA-based sequencing analysis
- Describe the utility of RNASeq data in characterizing tumors, including variants identified by RNASeq that conventional DNA sequencing failed to detect
- Highlight the Expressed Exome Capture Sequencing (EecSeq) method for using an RNA-based approach to analyze species without an annotated genome
- Answer your questions during the live broadcast!
This webinar will last for approximately 60 minutes.