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Overcoming challenges in cellular analysis: Multiparameter analysis of rare cells

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Overcoming challenges in cellular analysis: Multiparameter analysis of rare cells

Recorded 28 January 2015



There are a variety of challenges when studying rare cell types, primarily due to their very low abundance against a high background of other cells. Identifying ways to study these cell types  and better understand their roles in different human diseases is important to advance basic biomedical research—especially elucidating and characterizing disease mechanisms and targets—as well as in medical diagnostics and therapeutics.  With multiparameter capabilities and a very high analysis rate, flow cytometry is one of the technologies often utilized to analyze rare cells. During this webinar, the application of this technology to the isolation and analysis of rare cells will be discussed, with a focus on innate lymphoid cells (ILCs), circulating antigen-specific lymphocytes, and innate-like cells (such as natural killer T cells and circulating endothelial cells).

During this webinar viewers will be able to ask questions of the panelists and will learn about:

  • Advances in flow cytometry technologies that further enhance the capabilities for rare cell analysis
  • Studies utilizing multiparameter flow cytometry on human ILCs to interrogate their characteristics and immune function
  • How flow cytometry can be used to characterize the polyfunctionality of these very rare cells, and to investigate the role of such elements in diseases like multiple sclerosis.

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Speaker bios

Andrea Cossarizza, M.D., Ph.D.

University of Modena and Reggio Emilia School of Medicine
Modena, Italy

Dr. Cossarizza completed his M.D. at the University of Padova in Italy before going on to earn a Ph.D. in oncology at the University of Modena and University of Bologna, also in Italy. After specializing in clinical pathology at the University of Modena and Reggio Emilia, he obtained an associate professorship at the same school before spending time in the private sector as the founder of the academic spin off GeneMoRe, Italy. In 2005, Dr. Cossarizza was appointed professor in the international Ph.D. program at the University of Valencia, Spain, where he later became a research professor. In 2010, Dr. Cossarizza became full professor in pathology and immunology in the Faculty of Medicine at the University of Modena and Reggio Emilia. His overarching research focus is identifying the molecular and cellular basis for the involvement of the immune system in diseases and infections, including HIV/AIDS and sepsis, as well as its role in pathophysiological conditions related to aging and neurodegeneration. Dr. Cossarizza has particular experience in the development and use of new flow cytometric approaches in immunological research. In 2008, he won the Special Partec Prize for Excellence in Science at the ISAC World Congress in Budapest, Hungary.

David Cousins, Ph.D.

University of Leicester
Leicester, UK

Dr. Cousins pursued undergraduate and graduate training at University College London and King’s College London in the United Kingdom, respectively. Following receipt of his Ph.D., he stayed on at King’s College London first as a research fellow and then as a tenured lecturer. Since 2014, Dr. Cousins has been a professor of respiratory science at the University of Leister, UK, while also retaining a visiting professorship at King’s College London. In addition, he is a principal investigator at the Medical Research Council (MRC) & Asthma UK Centre in Allergic Mechanisms of Asthma and an honorary lecturer at Imperial College London. Dr. Cousins’ research interests include the study of specialized immune cells involved in inflammation in the lungs, particularly in patients suffering from asthma and allergies, including the recently discovered innate lymphoid cell. As part of his work, Dr. Cousins has developed and applied multicolor flow cytometry to the sorting and characterization of different immune cell types in the hope of gaining a deeper understanding of these cells, enabling the development of new and better therapies for respiratory diseases.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently, Dr. Sanders is the Director and Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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