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Monitoring immune function by imaging flow cytometry

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Monitoring immune function by imaging flow cytometry

Recorded 07 June 2017


The immune system plays a critical role not only in fending off pathogen attack, but also in cancer surveillance, and more recently as a tool in immunotherapy-based treatments. Immune cell functions are tightly regulated by essential transcription factors such as NF-κB and NFAT. Monitoring immune cell activity—including phenotyping immune cell subsets, tracking cell proliferation, and measuring cytokine production—can provide insights into the overall status of immune function in patients, particularly those undergoing immunosuppression after transplants, enduring cancer treatment, or suffering from autoimmune disease or other pathologies that affect the immune system. Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells. This technique enables quantitative image analysis of the intracellular localization of the signaling intermediaries NF-κB and NFAT as parameters of immune activity. This webinar will introduce viewers to the process of using IFC to determine subcellular localization of biomarkers, including a discussion of how IFC can help to assess the activity of transcription factors, or the drug-induced stimulation or inhibition thereof, in clinical samples.

During the webinar, viewers will:

  • Learn the process of using IFC to study transcription-factor signaling
  • Discover how IFC can help to determine the effects of drug inhibition or stimulation on immune function
  • Gain insight into preclinical and clinical applications of IFC.

This webinar will last for approximately 60 minutes

Speaker bios

Orla Maguire, Ph.D.

Roswell Park Cancer Institute
Buffalo, NY

Dr. Maguire is a senior cytometry specialist in the department of Flow and Image Cytometry at the Roswell Park Cancer Institute in Buffalo, New York. She obtained her Ph.D. from Ulster University in Northern Ireland, United Kingdom, studying the activity of nuclear-receptor transcription factor pathways in cancer. Her current research focuses on developing clinical applications for imaging flow cytometry, with special attention to transcription factor pathways. The goal of this research is to provide clinicians and researchers with useful information on the function of transcription factor pathways in immunophenotypically defined populations. She has published numerous original method manuscripts in this area, and is currently collaborating with hospital-based research groups and pharmaceutical companies worldwide studying transcription factors pathways in several diseases.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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