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Microscopy in Focus: The Art and Science of Image Quality

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Microscopy in Focus: The Art and Science of Image Quality

Recorded 07 March 2013



High resolution microscopy has become a widespread and ubiquitous technology in most life science laboratories and high quality images have become an essential part of many publications. Technology such as confocality can enhance image contrast by eliminating out of focus light, but this is often at the expense of signal intensity and increased phototoxicity. In this webinar our experts will discuss the benefits and challenges of confocality, compare and contrast it with other technologies such as deconvolution, and provide advice on which technology is best for which application.

During the webinar, our viewers will:

  • Obtain an overview of confocal microscopy and its application in basic research
  • Learn about technologies such as deconvolution, which can help to preserve maximum light input and provide better image quality
  • Receive best practice advice for obtaining the best quality images, regardless of the technique used
  • Have their questions answered live by our respected thought-leaders!

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Speaker bios

Jason Swedlow, Ph.D.

University of Dundee
Dundee, Scotland

Dr. Swedlow received his Ph.D. in biophysics from the University of California, San Francisco (UCSF) after completing a B.A. in chemistry at Brandeis University in Massachusetts. He joined the Wellcome Trust Biocentre at the University of Dundee in the United Kingdom following postdoctoral training at UCSF and Harvard Medical School in the United States. Dr. Swedlow is currently a professor of quantitative cell biology at the University of Dundee. His research interests are the mechanisms and regulation of chromosome segregation during mitotic cell division, and the development of software tools for accessing, processing, sharing, and publishing large scientific image datasets. Dr. Swedlow is a co-founder of the Open Microscopy Environment (OME), an international consortium that develops and releases open source software for biological imaging. He also co-founded Glencoe Software, Inc., which commercializes and customizes OME technology for use in biopharma and data publishing, and BioImagingUK, a consortium of U.K. imaging scientists who develop, use, or administer imaging solutions for life sciences research. In 2011, Dr. Swedlow was named Social and Overall Innovator of the Year by the BBSRC and in 2012 became a Fellow of the Royal Society of Edinburgh.

John Murray, M.D., Ph.D.

Indiana University
Bloomington, IN

Dr. Murray studied chemistry at Spring Hill College in Mobile, Alabama, and biochemistry and medicine at St. Louis University in Missouri, before completing an M.D., Ph.D. at the University of Pennsylvania in Philadelphia. Following postdoctoral training at the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom, and Stanford University in the United States, Dr. Murray joined the University of Pennsylvania Medical School. Since 2012, he has been a senior scientist in the Department of Biology at Indiana University in Bloomington, Indiana. The focus of Dr. Murray’s research is in understanding how the structures of proteins and macromolecular assemblies give rise to the behavior and properties of cells. Much of his work investigates assembly of the cytoskeleton and replication of the human pathogen Toxoplasma gondii, which infects an estimated one-quarter of the world population.

Paul C. Goodwin, M.Sc.

GE Healthcare
Issaquah, WA

Mr. Goodwin is the science director for Cell Analysis, part of the Research & Applied Markets business within GE Healthcare, located in the Seattle, Washington area. Mr. Goodwin received his Bachelor’s degree from the University of California, San Diego and his Master’s degree from the University of Washington in Seattle. He then began his career as a research technician in the Department of Pathology at the University of Washington, where he was introduced to the use of digital image processing and image analysis with light and electron microscopy. Mr. Goodwin was recruited by the Fred Hutchinson Cancer Research Center to create and manage the Image Analysis Core Facility where he developed a number of new imaging techniques. After 10 years he moved to Applied Precision in Issaquah, Washington. There he served various roles in advanced application development, product management, and R&D, before the company was purchased by GE. In addition to his role as science director, Mr. Goodwin is also an affiliate teaching associate in the Department of Comparative Medicine at the University of Washington. He is an expert in 3-D optics, deconvolution, superresolution microscopy, and instrument development. He lectures frequently and serves as commercial faculty for microscopy courses at the Marine Biological Laboratory, Woods Hole, Massachusetts and The Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently, Dr. Sanders is the Director and Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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