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Long-term volumetric imaging meets subcellular resolution: Lattice light-sheet microscopy comes of age

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Long-term volumetric imaging meets subcellular resolution: Lattice light-sheet microscopy comes of age

09 December 2020

12:00 p.m. ET

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Since it was first described and demonstrated in 2014, lattice light-sheet microscopy has been exciting the research community. This technology combines the gentleness of conventional light-sheet imaging with the high resolution of confocal microscopes, allowing living samples to be imaged over longer periods of time and with subcellular resolution. To create a lattice light-sheet image, the sample is illuminated perpendicular to the direction of observation so that only those areas of the sample in the focal plane are exposed to the damaging effects of light. Innovations have allowed for the generation of thinner light sheets, significantly improving resolution in z, while retaining the advantages of traditional light-sheet microscopy—fast volumetric imaging with minimal phototoxicity. The combination of both high spatial and temporal resolution, rapid imaging, and unrivaled gentleness makes lattice light-sheet microscopy the preferred choice for live-cell imaging. In this webinar, our experts will introduce the viewer to this enabling technology, highlighting its development, utility, and application.

During the webinar, viewers will:

  • Learn about the principles of lattice light-sheet microscopy and its advantages for 3D imaging of subcellular dynamics over time
  • Gain insights into the development process for this technique
  • See how first-time users have successfully implemented this technology to address critical questions in life sciences
  • Have the opportunity to ask questions during the live broadcast.

This webinar will last for approximately 60 minutes.

Speaker bios

Eric Betzig, Ph.D.

University of California, Berkeley/HHMI
Berkeley, CA

Dr. Betzig is professor of molecular and cell biology, the Eugene D. Commins Presidential Chair in Experimental Physics, and a Howard Hughes Medical Institute (HHMI) Investigator at the University of California, Berkeley. From 1988 to 1994, he ran a small laboratory at AT&T Bell Labs that worked on the development and application of near-field optics—an early form of superresolution microscopy. He then left science to work in the machine-tool industry, but returned 10 years later when he and friend Harald Hess built the first superresolution, single-molecule localization microscope in Hess’s living room. For this work, he was a corecipient of the 2014 Nobel Prize in Chemistry. From 2006 to the present, he has led a group at the Janelia Research Campus of HHMI focused on developing new imaging tools for biology, including lattice light-sheet microscopy for the 4D dynamic imaging of living systems, and adaptive optics to recover optimal imaging performance deep within aberrating tissues. He joined the Berkeley faculty in 2018.

Hjalmar Brismar, Ph.D.

KTH Royal Institute of Technology
Stockholm, Sweden

Dr. Brismar is a professor of biological physics at KTH Royal Institute of Technology in Stockholm, Sweden. He is director of the Cellular and Molecular Imaging platform at the Swedish research infrastructure Science for Life Laboratory and head of the national microscopy infrastructure. His laboratory develops imaging-based methods, primarily for applications in cellular metabolism and signaling. A specific focus of his research group is the Na+,K+-ATPase enzyme and its unconventional role in cellular signaling. Dr. Brismar completed his undergraduate degree in engineering physics at KTH, where he remained to complete his Ph.D. in the same field. He undertook his postdoctoral training in biomedical imaging at Harvard University.

Klaus Weisshart, Ph.D.

Carl Zeiss Microscopy GmbH
Jena, Germany

Dr. Weisshart is a product manager at Carl Zeiss Microscopy GmbH. He graduated with a degree in biology from the University of Constance and obtained his Ph.D. from the German Cancer Research Center in Heidelberg. He continued his work on DNA replication during research stays at Harvard Medical School in Boston, Massachusetts, and the Ludwig Maximilian University in Munich, Germany. In 2000, he joined Carl Zeiss and currently supports the product lines for fluctuation analysis, superresolution microscopy, and light-sheet microscopy.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently, Dr. Sanders is the Director and Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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