Lipids are extremely diverse molecules, but their diversity, structure, and exact function are not well understood. Precise determination of each molecular species of lipid is a prerequisite for understanding their functions in physiology and disease, and for discovering novel, bioactive lipid-derived mediators that may have therapeutic benefits. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a powerful method for analyzing lipid metabolites, providing insight into the structure and function of endogenous lipid metabolites that regulate inflammation and tissue homeostasis. Examples include the omega-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid, and precursors to the specialized proresolving mediators (SPMs), such as the resolvin, protectin, and maresin families. PUFAs are thought to be beneficial in maintaining tissue homeostasis and inflammation, while SPMs have demonstrated proresolving and anti-inflammatory actions as well as the ability to enhance microbial clearance by the immune system. Using targeted lipidomics systems, fatty acid–derived mediators can be comprehensively monitored to elucidate the role of omega-3 PUFA-derived mediators in controlling the inflammatory response and its resolution, highlighting their exciting potential for identifying targets for the treatment of inflammatory diseases.
During the webinar, the speakers will:
- Outline how the latest mass spectrometry techniques, such as Q-TOF and TripleQ, are being applied in lipid research
- Describe recent advances in understanding the role of omega-3 PUFAs in controlling the inflammatory response
- Explain the structural elucidation of SPMs derived from PUFAs during inflammation resolution
- Highlight recent results, using resolvin D4 as example, showing how SPMs reduce venous thrombosis burden and activate resolution of inflammation
- Answer your questions live during the broadcast.
This webinar will last for approximately 75 minutes.