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Innovations in Light Sheet Microscopy: Strategies and New Applications

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Innovations in Light Sheet Microscopy: Strategies and New Applications

Recorded 29 October 2014



Over the past 10 years, light sheet microscopy (or selective plane illumination microscopy, SPIM) has transformed the microscopy field, offering a faster, less phototoxic technique than conventional methods that can create true 3-D images. Ideal for observing living organisms and the cellular dynamics of biological systems, this method uses a unique illumination approach to achieve high penetration depths, fast imaging speeds, and subcellular-level resolution. Because a specimen is illuminated with a sheet of light rather than a focused laser beam, only regions directly exposed to light will fluoresce—creating minimal photo-induced tissue damage. In this webinar, the speakers will discuss their own uses of light sheet microscopy and provide insight into its different applications, including open source SPIM and the dynamic behavior of subcellular components within live specimens.

During the webinar, viewers will:

  • Learn about state-of-the-art light sheet microscopy techniques
  • Discover recent advances in the field
  • Hear how microscopists are applying the technology
  • Have their questions answered live by our expert panel!

To learn more about products or technologies related to this webinar, go to: www.andor.com/light-sheet-microscopy

Speaker bios

Thai Truong, Ph.D.

University of Southern California
Los Angeles, CA

Dr. Truong is a research scientist in the Translational Imaging Center at the University of Southern California, where he is developing novel imaging technologies and associated quantitative analytical tools for applications in developmental biology, neuroscience, and regenerative science. Dr. Truong obtained his Ph.D. from the University of California, Berkeley and received his postdoctoral training at both the Biological Imaging Center, California Institute of Technology and the Physics Department at the University of California, Berkeley. His recent research includes developing and applying light sheet imaging technology to the study of dynamic biological systems, such as the dorsal-ventral axis patterning in Drosophila embryos, and the interplay between form and function in zebrafish embryonic hearts.

Peter G. Pitrone, DipRMS

Dresden, Germany

Mr. Pitrone has been a microscopist and optical designer at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) in Dresden, Germany since 2011, where he is responsible for building Open Access Selective Plane Illumination Microscope (Open-SPIM) systems. Prior to his current role, Mr. Pitrone worked as a microscopy and imaging specialist at MPI’s Light Microscopy Facility for over five years, providing training and technical support on a wide variety of light microscope and laser scanning confocal microscope systems, including spinning disk confocal microscopy, multi-photon microscopy, structured illumination microscopy, aperture correlation microscopy, and total internal reflection fluorescence microscopy. He has earned both a TechRMS certification as well as a Diploma of Microscopy (DipRMS) from the Royal Microscopical Society. His current focus is on developing Open-SPIM systems to characterize the changes in gene expression patterns over time in developing Drosophila embryos in 3-D.

Orla Hanrahan, Ph.D.

Andor Technology
Belfast, Ireland

Dr. Hanrahan has worked with Andor Technology as an application specialist in life science for the past four and half years. Her role brings her in touch with all the latest developments and innovations in both camera technology and microscopy applications. Previous to this Dr. Hanrahan managed a microscopy facility in the Biochemistry Department at Trinity College, Dublin where she worked with a variety of microscopy setups including wide-field fluorescent microscopy, point scanning confocal microscopy, and spinning disk confocal microscopy. Her interest in microscopy stemmed from her Ph.D. in biochemistry, during which she gained experience with numerous microscopy techniques used in her graduate research.

Tianna Hicklin, Ph.D.

Washington, DC

Dr. Hicklin studied biology at Colorado State University for her undergraduate education before earning a Ph.D. in neuroscience from the University of Colorado Anschutz Medical Campus. Prior to joining Science/AAAS, she worked as a science writer intern for the University of Colorado’s Office of Media and Public Relations in Denver, Colorado and for Brookhaven National Laboratory’s Media and Communications Office in Upton, New York. Dr. Hicklin is currently the assistant editor for the Science/AAAS Custom Publishing Office.

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