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Improving tissue-sample profiling: The optimization and application of immunohistochemistry

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Improving tissue-sample profiling: The optimization and application of immunohistochemistry

Recorded 07 July 2015



Though it has been used for more than 70 years, immunohistochemistry (IHC) is still an essential research and diagnostic tool in many scientific laboratories. Understanding the basic principles underlying IHC and how to address the technical aspects of experimental design are key to producing high-quality, reproducible data. IHC is used in a variety of fields— from cancer diagnostics to neuroscience research—but some common advice can be applied across-the-board. Many variables are vital for generating valuable results and require optimization when designing IHC experiments, such as fixing tissue, choosing the proper antibodies, and defining the proper controls. In this webinar, we will hear from experts who will share their insights into the key aspects of assay design.

During the webinar, the speakers will:

  • Provide an overview of their immunohistochemistry methods
  • Highlight some best practices and common challenges in assay design
  • Discuss the applications of IHC in cancer research and neuroscience
  • Answer your questions live during the broadcast!

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Speaker bios

Nissi M. Varki, M.D.

University of California San DiegoLa Jolla, CA

Dr. Varki received her MBBS (M.D. equivalent) from Christian Medical College in Vellore, India, before completing a pathology residency at Creighton University in Omaha, Nebraska and St. John’s Mercy Medical Center, followed by a postdoctoral fellowship at Washington University in St. Louis, Missouri. She was Board Certified in Anatomic and Clinical Pathology and did her postdoctoral fellowship in immunology at Scripps Clinic in La Jolla, California before becoming an assistant professor at University of California, Los Angeles. Dr. Varki transferred to the University of California San Diego and is now professor of pathology and also serves as the director of the Histopathology Core Laboratories and the Mouse Phenotyping Services. She analyzes the comparative histopathology of genetically altered mice and models of human diseases, including cancer, inflammatory disorders, and microbial infections. She is investigating the role of glycosylated molecules in tumor progression and metastasis, tissue- and species-specific expression of lectin receptors that play a role in regulating host innate immune responses and inflammation, and the immunological mechanisms underlying chronic inflammation and cancer development.

Kevin D. Long, Ph.D.

EMD Millipore
Temecula, CA

Dr. Long completed his undergraduate degree in zoology at University of Maryland, followed by a Ph.D. in biology at the University of California, Santa Barbara. Throughout his career, he has held positions in which he conducted research investigating the sensory neurobiology underlying important animal behaviors and preferences, including studies on the fish and mammalian visual system and inborn preferences for avian maternal calls. Much of this work was done while developing novel antibody and in situ hybridization techniques. In 2001, he joined the antibody company, Chemicon International (now part of EMD Millipore), to help develop their nascent neuroscience program. Currently, Dr. Long leads the Technical Content Marketing Team at EMD Millipore and has roles in strategic product development based on his extensive knowledge of EMD Millipore’s current portfolio. Additionally, he leads EMD Millipore’s Neuroscience product group and presents training seminars. A lifelong teacher, Dr. Long still designs science curricula ranging from K–5 to medical professional education. 

Tianna Hicklin, Ph.D.

Washington, DC

Dr. Hicklin studied biology at Colorado State University for her undergraduate education before earning a Ph.D. in neuroscience from the University of Colorado Anschutz Medical Campus. Prior to joining Science/AAAS, she worked as a science writer intern for the University of Colorado’s Office of Media and Public Relations in Denver, Colorado and for Brookhaven National Laboratory’s Media and Communications Office in Upton, New York. Dr. Hicklin is currently the assistant editor for the Science/AAAS Custom Publishing Office.

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