Resolving the spatial distribution of the human proteome at a subcellular level greatly increases our understanding of human biology and disease. A high-resolution map of the human cell has been generated—part of the Human Protein Atlas database—that provides the in situ localization of 12,036 human proteins at a single-cell level, covering 30 subcellular structures, and enabling 14 major organelle proteomes to be defined. The high spatial resolution of the data has allowed the identification of novel protein components in all major organelles, as well as the characterization of fine cellular structures such as the cytokinetic bridge and nuclear bodies. An integrative approach to data generation includes strict validation criteria using gene silencing, paired antibodies, and fluorescently tagged proteins. The Cell Atlas reveals that approximately half of all proteins localize to multiple compartments and that many proteins show cell-to-cell variation in terms of protein abundance or spatial distribution. In this webinar, we will introduce the new Human Cell Atlas, outlining how it is being used to define the spatiotemporal organization of the human proteome at a subcellular level.
During the webinar, viewers will learn about:
- The generation of the Human Cell Atlas and how its data was validated
- The process for identification and characterization of the organelle proteomes
- The identification and localization of proteins, particularly those showing complex distribution and cell-to-cell variations in expression.
This Webinar will last for approximately 60 minutes.