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Getting the best data from your cells: From tissue culture to final analysis

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Getting the best data from your cells: From tissue culture to final analysis

Recorded 03 May 2017


From tissue culture to final analysis, cell culture is a group of techniques used in the majority of life science laboratories. There are numerous standard methodologies, but many of the techniques used are continuously changing and evolving. From cell preparation and growth to final analysis, the cell culture workflow encompasses many areas of expertise, making it difficult for a researcher to be an authority in all of them. Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing scientific research. Join this webinar to learn the latest tips and tricks of the trade from the experts.

Attendees can expect to learn about:

  • Proper preparation of cells for culturing
  • Optimizing growth conditions through choice of media and growing conditions
  • How to best handle cells for smoother analysis and reproducible results.

This webinar will last for approximately 60 minutes

Speaker bios

Christopher Korch, Ph.D.

University of Colorado Anschutz Medical Campus
Aurora, CO

Dr. Korch received his Ph.D. in genetics from the University of California, Davis before undertaking postdoctoral training there and at Louis Pasteur University in Strasbourg, France. He has worked in many academic settings during his illustrious career, including Umeå University and Stockholm University in Sweden and the University of Colorado in the United States. He is currently assistant clinical professor (semiretired) in the Department of Medicine at the University of Colorado School of Medicine, Anschutz Medical Campus in Aurora, Colorado. His research has focused on different aspects of gene structure, function, and regulation in various microorganisms, filamentous fungi, and human cells. Since 1996, he has directed the University of Colorado’s Cancer Center’s DNA Sequencing & Analysis Core, using DNA sequencing and profiling to confirm the identity of numerous authentic and false human cell lines and the mutations they carry. In 2012, he led a major study on the identity of endometrial and ovarian cancer cell lines, showing that there was extensive contamination, redundancy, and misidentification among these cell lines, and raising questions about the validity of many publications using them. Since his semiretirement at the end of 2012, Dr. Korch has been involved in identifying several additional misidentified/false cell lines.

Karina Eterovic, Ph.D.

The University of Texas MD Anderson Cancer Center
Houston, TX

Dr. Eterovic is an associate professor and head of the Genomic Laboratory at the Institute for Personalized Cancer Therapy at MD Anderson Cancer Center in Houston, Texas. Her current research is focused on personalized cancer treatment using next-generation sequencing to characterize cancer-related genomic aberrations, helping physicians to determine the best treatment for patients or identify potential drug resistance. She is also director of the Characterized Cell Line Core (CCLC) Facility at MD Anderson. Cell line validation is a critical issue for both scientific publications and grant applications, and her core provides rapid and cost-effective short tandem repeat (STR) fingerprinting for investigators inside and outside MD Anderson. In addition to STR, CCLC also performs mutational analysis for established cell lines using MassARRAY technology. Dr. Eterovic has authored and coauthored numerous publications in high-impact journals reporting the significance of her work in the field of personalized cancer treatment.

Richard M. Neve, Ph.D.

Gilead Sciences Inc.
Foster City, CA

Dr. Neve has 20 years of experience in the life sciences, spanning both academia and industry. After earning a Ph.D. studying the ERBB2 oncogene at the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland, he was a postdoctoral fellow at the University of California, San Francisco, and then at Lawrence Berkeley National Laboratory, where he utilized genomics to identify therapeutic targets in breast cancer. Subsequently, as a scientist in Discovery Oncology at Genentech in San Francisco, he ran a target discovery group. Dr. Neve is currently a senior scientist in the Oncology group at Gilead Sciences in Foster City, California.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently, Dr. Sanders is the Director and Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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