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Every cell has its place: In situ sequencing of tissue samples at single-cell resolution

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Every cell has its place: In situ sequencing of tissue samples at single-cell resolution

22 April 2020

12:00 p.m. ET

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Single-cell RNA sequencing (scRNA-seq) has revolutionized the understanding of tissue complexity by dissecting transcriptomic heterogeneity and revealing previously unknown cell types and states. However, scRNA-seq does not allow the visualization of spatial organization of single cells within the tissue. In order to overcome this limitation, spatial gene expression profiling technologies have emerged that aim to localize molecularly defined cell types within the morphological context. In situ sequencing (ISS) can rapidly analyze and visualize the expression of hundreds of genes within morphologically intact tissue samples at single-cell resolution. ISS helps researchers to understand complex biological and pathological mechanisms and to localize and validate new drug targets.

In this webinar, we will outline the benefits of ISS and showcase how it can complement spatial transcriptomics technology in the task of unraveling the role of amyloid-ß plaques in the neurogenerative process of Alzheimer’s disease.

During this webinar, the speakers will:

  • Introduce ISS technology and how it enables simultaneous spatial analysis of hundreds of genes in tissue samples at single-cell resolution
  • Show how ISS has helped shed light on the role of amyloid-ß plaques in Alzheimer’s disease
  • Discuss applications and performance of ISS in other research areas
  • Answer your questions during the live broadcast.

This webinar will last for approximately 60 minutes.

Speaker bios

Malte Kuhnemund, Ph.D.

Stockholm, Sweden

Dr. Kühnemund obtained a Master’s in biochemistry and cell biology at the Braunschweig University of Technology (Germany) and Lund University (Sweden). He then joined the Nilsson and Landegren labs in Sweden, where he obtained his Ph.D. in molecular medicine from Uppsala University and the Science for Life Laboratory (Sweden). His work on single-molecule analysis and sequencing technologies is now part of the technology portfolio of CARTANA, an in-situ-sequencing (ISS) company spun out from the Nilsson lab. Dr. Kühnemund is a cofounder of CARTANA and head of R&D at the company, which makes ISS technology available to the global research community.

Wei-Ting Chen, Ph.D.

VIB-KU Leuven Center for Brain & Disease Research
Leuven, Belgium

Dr. Wei-Ting Chen is a postdoctoral scientist in the Laboratory for Research of Neurodegenerative Diseases led by Bart De Strooper at VIB-KU Leuven, Belgium. She received her Ph.D. from Academia Sinica in Taipei, Taiwan, for characterizing molecular mechanisms of several familial Alzheimer’s disease mutations. At VIB-KU Leuven, she focuses on the molecular responses of cells in the microenvironments of Alzheimer’s amyloid plaques. Dr. Chen uses spatial 'omics approaches to study the brains of animal models and human brains. Her team incorporates the latest advances in the rapidly evolving field of single-cell biology, using spatial resolution techniques to further Alzheimer’s research and to identify new, critical molecular signaling pathways for therapeutic developments.

Jackie Oberst, Ph.D.

Washington, DC

Dr. Oberst did her undergraduate training at the University of Maryland, College Park, and her Ph.D. in Tumor Biology at Georgetown University, Washington D.C. She combined her interests in science and writing by pursuing an M.A. in Journalism from the Philip Merrill College of Journalism at the University of Maryland, College Park. Dr. Oberst joined Science/AAAS in 2016 as the Assistant Editor for Custom Publishing. Before then she worked at Nature magazine, the Howard Hughes Medical Institute, The Endocrine Society, and the National Institutes of Mental Health.

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