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Webinar Technology

Every cell has its place: In situ sequencing of tissue samples at single-cell resolution

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Every cell has its place: In situ sequencing of tissue samples at single-cell resolution

22 April 2020

12:00 p.m. ET

Register now!


Single-cell RNA sequencing (scRNA-seq) has revolutionized the understanding of tissue complexity by dissecting transcriptomic heterogeneity and revealing previously unknown cell types and states. However, scRNA-seq does not allow the visualization of spatial organization of single cells within the tissue. In order to overcome this limitation, spatial gene expression profiling technologies have emerged that aim to localize molecularly defined cell types within the morphological context. In situ sequencing (ISS) can rapidly analyze and visualize the expression of hundreds of genes within morphologically intact tissue samples at single-cell resolution. ISS helps researchers to understand complex biological and pathological mechanisms and to localize and validate new drug targets.

In this webinar, we will outline the benefits of ISS and showcase how it can complement spatial transcriptomics technology in the task of unraveling the role of amyloid-ß plaques in the neurogenerative process of Alzheimer’s disease.

During this webinar, the speakers will:

  • Introduce ISS technology and how it enables simultaneous spatial analysis of hundreds of genes in tissue samples at single-cell resolution
  • Show how ISS has helped shed light on the role of amyloid-ß plaques in Alzheimer’s disease
  • Discuss applications and performance of ISS in other research areas
  • Answer your questions during the live broadcast.

This webinar will last for approximately 60 minutes.

Speaker bios

Bart De Strooper, M.D., Ph.D.

VIB-KU Leuven Center for Brain & Disease Research
Leuven, Belgium

Dr. DeStrooper graduated from KU Leuven, first as a Doctor of Medicine (1985) and then as a Master’s in biomedical sciences (1987). After obtaining his doctorate in 1991 and completing postgraduate studies in 1999 under the supervision of Fred van Leuven and Carlos Dotti (European Molecular Biology Laboratory, Germany), in which he studied presenilins in Alzheimer’s disease (AD), he started an independent research laboratory at KU Leuven and VIB. His multidisciplinary, international research group has grown to 30 people, and his lab studies both Alzheimer’s and Parkinson’s disease. His research spans biochemistry and enzymology of γ-secretase; microRNA biology and expression deregulation; genetic and transcriptomic analysis of AD patients’ brains; generation of novel humanized mouse models for AD; mitochondrial biology; and the development of nanobodies against therapeutically relevant targets. Currently, Dr. DeStrooper is pioneering the application of single-cell genomics-transcriptomics and spatial transcriptomics to the study of Alzheimer’s disease. Since 2017 he has been founding director of the UK Dementia Research Institute.

Malte Kuhnemund, Ph.D.

Solna, Sweden

Dr. Kühnemund obtained a Master’s in biochemistry and cell biology at the Braunschweig University of Technology (Germany) and Lund University (Sweden). He then joined the Nilsson and Landegren labs in Sweden, where he obtained his Ph.D. in molecular medicine from Uppsala University and the Science for Life Laboratory (Sweden). His work on single-molecule analysis and sequencing technologies is now part of the technology portfolio of CARTANA, an in-situ-sequencing (ISS) company spun out from the Nilsson lab. Dr. Kühnemund is a cofounder of CARTANA and head of R&D at the company, which makes ISS technology available to the global research community.

Jackie Oberst, Ph.D.

Washington, D.C.

Dr. Oberst did her undergraduate training at the University of Maryland, College Park, and her Ph.D. in Tumor Biology at Georgetown University, Washington D.C. She combined her interests in science and writing by pursuing an M.A. in Journalism from the Philip Merrill College of Journalism at the University of Maryland, College Park. Dr. Oberst joined Science/AAAS in 2016 as the Assistant Editor for Custom Publishing. Before then she worked at Nature magazine, the Howard Hughes Medical Institute, The Endocrine Society, and the National Institutes of Mental Health.

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