Cell culture—the controlled growing of cells outside their natural environment—may be commonplace in molecular biology laboratories, but one thing that strikes fear in both novices and experts using these techniques is contamination. Whether it occurs via chemicals (impurities in media, sera, and water) or biological components (bacteria, viruses, and mycoplasma), contamination can bring research to a halt, wasting both time and money and possibly raising doubts about the validity of a laboratory’s findings. Studies have shown that up to 30% of animal cell cultures are contaminated by either microorganisms or other cells.While no researcher is immune to this common problem, an introduction to and/or refresher on good aseptic techniques can help reduce the occurrence of contamination and possibly its severity. This webinar will be a master class for all those who perform primary and immortalized cell culture. It will discuss best practices and common pitfalls, with a special section dedicated to the dangers of contamination and ways to avoid it. An additional section will be devoted to protein expression in suspension. This webinar should be equally beneficial to both novices and experts in cell culture.
During the webinar, the speakers will:
- Provide an overview of cell-culture techniques
- Discuss the different types of contamination
- Offer pointers on how to reduce contamination, including areas where even most good laboratories go wrong
- Answer viewer questions during the live broadcast.
This webinar will last for approximately 60 minutes.