Transfection of cells in culture is a key technique in the molecular and cell biology toolbox. Toxicity and efficiency are the factors that have the biggest impact on the success of transfection experiments and can significantly affect important downstream applications. In this webinar, we discuss the key steps and protocol considerations for performing high-quality transfections on a consistent basis, taking into consideration variables such as cell type, culture conditions, reagent concentrations, and other critical parameters. Drawing attention to these factors will ultimately lead to better downstream results for gene expression and other emerging applications, such as CRISPR/Cas9 genome editing, virus production, and cell-line development for biotherapeutic protein production. During the webinar, the speakers will:
During the webinar, the speakers will:
- Introduce the most critical factors to consider in any transfection experiment
- Discuss optimization procedures for a range of transfection applications, including recombinant protein production, CRISPR-based gene editing, and virus production
- Highlight current trends and new applications in transfection
- Answer your questions live during the broadcast!
This webinar will last for approximately 60 minutes.