This webinar was originally scheduled for broadcast on June 8, but was postponed due to technical issues.
Extracellular vesicles (EVs) such as exosomes (70 nm – 160 nm in diameter) and microvesicles (100 nm – 1,000 nm diameter) can be harvested from cell-culture supernatants and from all bodily fluids. Current standard techniques to visualize, quantify, and characterize EVs are electron microscopy, nanoparticle tracking analyses, and dynamic light scattering. To further characterize and discriminate EVs, more exact high-throughput technologies to analyze their surface are highly desired. Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm – 500 nm, a relatively new flow-cytometric method—called “imaging flow cytometry”—allows for the analysis of EVs smaller than 300 nm. This webinar will introduce viewers to the challenges, limitations, and pitfalls of flow cytometry-based EV analysis, and to the imaging flow cytometry methodology. Also covered will be techniques for analyzing exosomes, microvesicles, and apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.
During the webinar, viewers will learn about:
- Using an eGFP-positive biological calibrator to optimize imaging flow cytometry acquisition and analysis parameters
- The importance of appropriate controls when using fluorescent dyes and antibodies
- The value that imaging flow cytometry brings to multiparametric EV surface analysis.
The webinar will last approximately 60 minutes.
To learn more about products or technologies related to this webinar, go to: www.emdmillipore.com/amnis