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Adventures in single-cell RNA transcriptomics

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Adventures in single-cell RNA transcriptomics

25 April 2019

12:00 p.m. ET

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Single-cell RNA sequencing (RNA-seq) provides a powerful tool for the interrogation of gene expression within individual cells. By revealing cell-specific information not accessible through next-generation sequencing of heterogeneous cell populations, single-cell transcriptomics offers the potential to answer important questions about rare, disease-associated cells—such as circulating tumor cells—or about distinct cell types and lineages in healthy tissues. Despite its strong potential, single-cell transcriptomics faces several challenges, including working with the minute amounts of RNA typically present in individual cells, generally in the low-picogram range, as well as the potential loss of molecular complexity, particularly the loss of low-abundance transcripts when preparing sequencing libraries. In this webinar we’ll explore the exciting new field of single-cell RNA sequencing and discover some of the methods scientists are applying to unlock the secrets hidden in the transcriptomes of normal and diseased cells.

During the webinar, our speakers will:

  • Outline the advantages and challenges of working with single cells
  • Describe cutting-edge methodologies for performing single-cell RNA-seq
  • Illustrate applications for single-cell RNA-seq that answer previously intractable questions
  • Answer your questions during the live broadcast.

This webinar will last for approximately 60 minutes.

Speaker bios

Simone Picelli, Ph.D.

Institute of Molecular and Clinical Ophthalmology
Basel, Switzerland

Dr. Picelli currently works as team leader in the Single-Cell Genomics Platform at the Institute of Molecular and Clinical Ophthalmology Basel in Basel, Switzerland. He obtained his Ph.D. in biotechnology from the University of Padova (Italy) in 2006 before moving to Stockholm, Sweden for a postdoctoral position at the Karolinska Institute. After a short spell at the German Cancer Research Center in Heidelberg, he returned to Stockholm in 2012 for a second postdoc at the Ludwig Institute for Cancer Research in the group of Rickard Sandberg. There he developed the Smart-seq2 technique and an in-house version of Tn5 transposase for single-cell library preparation. In 2015, he joined the Eukaryotic Single-Cell Genomics facility opened by Rickard Sandberg and Sten Linnarsson at the Science for Life Laboratory (also in Stockholm). Dr. Picelli is currently working on new technologies to improve data quality, increase throughput, and reduce the cost of single-cell RNA sequencing.

Spyros Darmanis, Ph.D.

Chan Zuckerberg Biohub
San Francisco, CA

Dr. Darmanis carried out his doctoral research in Ulf Landegren’s laboratory at Uppsala University in Sweden, where he developed high-performance, multiplexed protein assays and applied them to identify putative protein biomarkers that could serve as early diagnostic indicators of cancer and other diseases. As a postdoctoral fellow in Stephen Quake’s laboratory at Stanford University, California, he carried out single-cell protein and RNA analyses to characterize the diversity of cell types in adult and fetal human brain. At CZ Biohub, he is creating tools to study different molecular entities in single cells in high-throughput and cost-effective ways, with a particular interest in studying the heterogeneity of human tumors and their interactions with the tumor microenvironment.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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