NGS Library Preparation
ThruPLEX Plasma-seq is powered by ThruPLEX chemistry to generate high performance next-generation sequencing (NGS) libraries from cell-free DNA isolated from plasma. The chemistry is optimized specifically for cell-free DNA to maximize the library complexity and to preserve the guanine-cytosine (GC) representation of the input DNA. Starting from less than 1 ng to 30 ng of cell-free DNA, ThruPLEX Plasma-seq generates indexed Illumina NGS libraries that are highly reproducible and consistent in performance between replicates and across different input amounts. ThruPLEX Plasma-seq is ideally suited for applications such as liquid biopsy, circulating tumor DNA (ctDNA) analysis, targeted sequencing, and noninvasive prenatal testing (NIPT).