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New Product

Running Buffer

For info: 800-766-7000
Fisher Scientific

The new Fisher BioReagents FastRun Tris SDS PAGE Running Buffer provides comparable or better resolution and an increased molecular weight separation range of proteins when compared to traditional Tris-glycine-sodium dodecyl sulfate (SDS) buffer. The new buffer system also reduces the number of Tris-glycine polyacrylamide gel compositions required to resolve a protein, saving researchers time and money. Tris-glycine mini-gels (precast or homemade) prepared with the conventional buffer system are typically run at 125 V. The buffer system heats up at high voltages, which in turn heats the gel, resulting in a breakdown of the protein bands and loss of resolution. The Fisher BioReagents FastRun buffer can be run at a higher voltage (200 V recommended), because it does not generate excessive heat. The result is a significant improvement in run times. In an example, gels run with the new buffer system took only 25 minutes, compared to 90-minute run times with the traditional reagents.

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