miRNA Profiling Assays
A new range of multiplex microRNA (miRNA) profiling assays is now available for high-throughput validation of miRNA biomarkers, based on the new Firefly particle technology. The new Multiplex Cellular miRNA Assay and Multiplex Circulating miRNA Assay allow researchers to rapidly and cost-effectively profile up to 68 miRNAs in each well of a 96-well plate, with readout on a standard flow cytometer. These assays offer an easier workflow for larger sample numbers compared to alternative methods such as quantitative polymerase chain reaction (qPCR), microarray, and sequencing, which can be resource intensive or require challenging data analysis. The Multiplex Circulating miRNA Assay enables profiling of miRNAs from small volumes of crude biofluids including serum and plasma, with no need for RNA purification, while the Multiplex Cellular miRNA Assay is optimized to work with purified RNA. The Multiplex Circulating miRNA Assay is particularly suited for studies targeting low-abundance miRNAs or studies in which the amount of each sample is limiting.