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New Product

HDR Plasmid Donor Kits

Dharmacon

For info: 800-235-9880
dharmacon.horizondiscovery.com

The Edit-R HDR Plasmid Donor Kits enable you to easily create a plasmid donor for insertion of either the mKate2 or enhanced green fluorescent protein (EGFP) fluorescence reporters, or a custom gene sequence of your choice, into almost any genomic location. Gene engineering using CRISPR/Cas9 relies on two pathways for repair of double-strand breaks in DNA: nonhomologous end joining (NHEJ) and homology-directed repair (HDR). Precise repair of DNA in order to generate a specific mutation or insert or remove a desired sequence requires the HDR pathway, and depends on the availability of a DNA donor template that can be used to repair the DNA break. The kits enable fast, efficient assembly of a DNA donor plasmid, and contain validated protocols for design, assembly, and confirmation of the plasmid.

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