Oxford Gene Technology
FFPE DNA Repair Mix
The SureSeq FFPE DNA Repair Mix is optimized to repair a broad range of damage in formalin-fixed, paraffin-embedded (FFPE)–derived DNA. Fixation processes significantly damage nucleic acids, and long storage times can produce problematic artifacts. In addition, many FFPE samples are often irreplaceable, and there is a real need to reduce DNA input for NGS. On the other hand, hybridization-based approaches eliminate PCR bias—providing much more reliable data—but require higher DNA inputs. The SureSeq mix increases the success and accuracy of hybridization-based NGS for FFPE samples by repairing damage such as nicks and gaps, oxidized bases, blocked 3' ends, and deamination of cytosine to uracil. Repairing this damage enables reduction of the amount of required starting material down to as little as 100 ng, while delivering excellent sequencing data for the utmost confidence in variant calling.