Innova Biosciences FlexLISA kits are designed for the development and optimization of sandwich ELISA assays to detect the presence of any antigen with a high affinity for any antibody pair in complex samples (serum, plasma, urine, and more). Benefits of the FlexLISA kit include easy antibody labeling via Lightning-Link technology, plus cost-effectiveness, as 40 times less capture antibody is used. Researchers can use their antibodies of choice and conjugate up to three capture and three detection antibodies. The kits use a rapid, one-step protocol, employing preblocked microtiter well strips coated with biotin. The FlexLISA assay is run by adding the sample and the antibody mix to the wells, incubating for 1 hour, washing, then reading the assay plate. FlexLISA is ideal for quick, reliable ELISA assay development, antibody pair screening, and ELISA assay optimization.
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