Gibson Assembly Cloning allows for the insertion of one or more DNA fragments into virtually any location of any vector without the need for compatible restriction sites. In a single round of cloning, join multiple DNA fragments (1–15) to create seamless constructs without subcloning. With easy reaction setup and less than 5 min of hands-on time, go from DNA fragments to transformation-ready constructs in an hour or less. Gibson Assembly Cloning achieves cloning efficiencies of up to 95%. Three kits are available: Gibson Assembly HiFi 1-Step Kit, Gibson Assembly Ultra Kit, and Gibson Assembly Site-Directed Mutagenesis Kit.