The Mesolens microscope makes possible for the first time the imaging of relatively large biomedically important specimens such as embryos, brain areas, or tumors, with full 3D recording of many thousands of cells. The microscope’s innovative design includes a novel apochromatic lens with an aperture-to-magnification ratio far in excess of conventional lenses used by other manufacturers. This low-magnification lens, with its exceptionally high numerical aperture, can image an entire specimen while retaining subcellular detail, thus removing the need for image stitching. The focus motor enables the Mesolens to provide highly accurate imaging in the z-axis. The leading-edge repeatability and precision of the microscope stage ensures that the correct position of the specimen is obtained and maintained throughout the entire imaging process.