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The power and purpose of multiplexing: Applications in microscopy imaging and flow cytometry

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It was not that long ago that scientists were limited to just one or two fluorescent dyes for tissue imaging or flow cytometry analysis. Following significant technological advancements over the past decade, multiplexing in now becoming the norm, allowing for the observation and analysis of upwards of 30 to 40 elements within a sample in the same experiment. Thanks to highly engineered optical interference filters and breakthroughs in light sources, many related objects and processes can be observed almost simultaneously, often in situ, saving time and money while generating more meaningful and reliable results. This first part in an ongoing series explains the foundations of multiplexing and its applications for imaging and flow cytometry.

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