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Illumination and clarity in multiplexing: Leveraging advanced light sources and detection devices



Multiplexing allows scientists to observe and follow as many as 30 to 40 different biological molecules in a cell at the same time with unprecedented precision and accuracy. It is invaluable in a range of biological applications including flow cytometry and microscopy, enabling dramatic advancements in our understanding of the movement, mechanism of action, degradation, and interaction of multiple molecules. Integral to multiplexing is the availability of reliable, advanced light sources and detection devices, without which the presence and movement of the molecules of interest would be invisible. Recent advances in detectors (including cameras) and light sources (such as LEDs and lasers)—both discussed in this article—have enhanced the power, reliability, efficacy, and ease of multiplexing.


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