Special Online Collection: Single Molecules

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Single MoleculesIn the 25 May 2007 issue, Science and it online companion Signal Transduction Knowledge Environment (STKE) explore recent advances in single-molecule techniques that move us ever closer toward a quantitative and mechanistic understanding of cellular life. Review articles in Science describe the role of "in silico" experiments in the quest to determine the mechanical properties of proteins, the power of combining molecular cell biology with single-force spectroscopy to explore eukaryotic cell adhesion, and the physical concepts that have pushed fluorescence microscopy to the nanoscale. In STKE, Perspective articles highlight the application of x-ray crystallography and nuclear magnetic resonance studies, microfluidic technology, and statistical algorithms to the analysis of signaling events at the level of single cells and individual molecules.

In Science

INTRODUCTION

Lights, Camera, Action  >
V. Vinson and G. Chin

REVIEWS

Single-Molecule Experiments in Vitro and in Silico  >
M. Sotomayor and K. Schulten
Forces and Bond Dynamics in Cell Adhesion  >
E. A. Evans and D. A. Calderwood
Far-Field Optical Nanoscopy  >
S. W. Hell

In Science's Signal Transduction Knowledge Environment

EDITORIAL GUIDE

Focus Issue: Single Molecules--Zooming In for a Closer Look  >
J. F. Foley and N. R. Gough
Technical advances allow analysis of signaling events at the level of single cells and individual molecules.

PERSPECTIVES

The Impact of X-ray Crystallography and NMR on Intracellular Calcium Signal Transduction by EF-Hand Proteins--Crossing the Threshold from Structure to Biology and Medicine  >
W. J. Chazin
Analysis of EF-hand proteins in complexes with their targets may define the structural basis for functional consequences.

PERSPECTIVES

Parsing the Motion of Single Molecules--A Novel Algorithm for Deconvoluting the Dynamics of Individual Receptors at the Cell Surface  >
I. Ghosh and M. J. Wirth
A new statistical method helps distinguish a protein moving with a purpose from a protein on a random walk.
Microfluidic Devices for the Analysis of Single Cells--Leaving No Protein Uncounted  >
M. Navratil, C. E. Whiting, E. A. Arriaga
A microfluidic device counts the number of low-abundance proteins in a single cell.