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Direct Allelic Variation Scanning of the Yeast Genome
Elizabeth A. Winzeler, Dan R. Richards, Andrew R. Conway, Alan L. Goldstein, Sue Kalman, Michael J. McCullough, John H. McCusker, David A. Stevens, Lisa Wodicka, David J. Lockhart, and Ronald W. Davis
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Supplementary Material
Fig. 3. Inheritance of DNA in 10 segregants. Tick colors are as described for Fig. 2. The
data are superimposed on a diagram showing the probable location of chromosomal breakpoints, calculated as described in the text. Light red segments show regions of probable YJM789 inheritance, while dark green segments show probable S96 inheritance. Arrows indicate the known locations of genes. lys2 LYS5 MATa and pdr5 were all inherited from YJM789 while ho was inherited from S96. All segregants except 1a (ho pdr5 lys5 MATa), 1b (ho::hisG MATa
), 1c (ho lys2 pdr5 lys5 MATa, and 1d (ho::hisG lys2 MATa) are (ho lys2 pdr5 MATa.
Fig. 4A. Calculated probability of random segregation for the 16 chromosomes in the Saccharomyces genome. The y-axis (log base 10) indicates the probability of random segregation calculated using a binomial distribution. The names and locations of open reading frames (taken from SGD, (16)) inside the intervals with the lowest probability
of random segregation (10 out of 10 = (1/2)10) are shown and are shaded in gray, except for those surrounding HO.
Figure 4B. Empirical and theoretical segregation distributions. Of the 413 total intervals (continuous chromosomal regions of inheritance across all segregants), 377 were at least 50 cM from all mapped loci. The histogram shows the number of these intervals observed with each S96:YJM789 segregation ratio. The curve is the expected number of intervals for each ratio, according to the binomial distribution.
