Specification of Drosophila Hematopoietic Lineage by Conserved Transcription Factors Tim Lebestky, Ting Chang, Volker Hartenstein, and Utpal Banerjee

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Web fig. 2. (A) A circulating crystal cell from a Bc/+ third-instar larva, showing characteristic melanization of crystalline inclusions (black). (B) Lz protein expression (green) in same crystal cell as (A), showing that Lz expression is maintained in mature crystal cells. (C and D) GFP expression in circulating hemocytes of a third-instar lz-gal4; UAS-GFP larva. (C) A circulating crystal cell expressing GFP. (D) A circulating plasmatocyte expressing GFP because of perdurance of the GFP protein (see text). Scale bar, 4 mm. (E to H) Circulating hemocytes from a third-instar lz-gal4; UAS-lacZ larva; b-gal expression (green) and Croquemort expression (red) are indicated. Scale bar, 8 mm. A single circulating plasmatocyte expresses ?-Gal (E) and Croquemort (F). A single crystal cell expresses b-Gal (G) but not Croquemort (H).

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Web fig. 3. Identical 1-?m confocal sections of a stage 12 lz-gal4/+; UAS-GFP; gcm-lacZ^rA87 embryo. (A) gcm (green) is expressed in the majority of hemocyte precursors. (B) Arrow denotes lz-GFP-expressing (red) crystal cell precursors. (C) Merged image of (A) and (B). gcm and lz-GFP do not colocalize in the crystal cell precursors, but they do colocalize in a small number of plasmatocytes migrating out of the bilateral Lz clusters (arrowhead). These few lz-GFP expressing plasmatocytes never express Lz protein (see text).

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