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Abstract
Full Text
Mechanical Rotation of the c Subunit Oligomer in ATP Synthase (F0F1): Direct Observation
Yoshihiro Sambongi, Yuko Iko, Mikio Tanabe, Hiroshi Omote, Atsuko Iwamoto-Kihara, Ikuo Ueda, Toshio Yanagida, Yoh Wada, and Masamitsu Futai

Supplementary Material

To view these movies, download a QuickTime viewer.

    Real-time video images of rotating actin filaments connected to the c subunit of the engineered Escherichia coli F0F 1. The images in Movie 2 were recorded with a 1.6 magnification,compared to Movies 1, 3, and 4.

  • Movie 1
    A filament (2.2 mm) rotating with the axis at one end of the filament. Running time: 24 s.

  • Movie 2
    A filament (1.5 mm) rotating with the axis at one end. Running time: 14 s.

  • Movie 3
    A filament (2.2 mm) with the axis close to the center of the filament. Running time: 9 s.

  • Movie 4
    A filament (2.8 mm) with the axis close to the center. Running time: 18 s.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)