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Mechanical Rotation of the c Subunit Oligomer in ATP Synthase (F0F1): Direct Observation
Yoshihiro Sambongi, Yuko Iko, Mikio Tanabe, Hiroshi Omote, Atsuko Iwamoto-Kihara, Ikuo Ueda, Toshio Yanagida, Yoh Wada, and Masamitsu Futai
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Supplementary Material
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Real-time video images of rotating actin filaments connected to the c subunit of the engineered Escherichia coli F0F
1. The images in Movie 2 were recorded with a 1.6 magnification,compared to Movies 1, 3, and 4.
- Movie 1
A filament (2.2 mm) rotating with the axis at one end of the filament. Running time: 24 s.
- Movie 2
A filament (1.5 mm) rotating with the axis at one end. Running time: 14 s.
- Movie 3
A filament (2.2 mm) with the axis close to the center of the filament. Running time: 9 s.
- Movie 4
A filament (2.8 mm) with the axis close to the center. Running time: 18 s.
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