While conventional RNA sequencing allows comprehensive transcriptome analyses at steady state, its utility for probing transcriptional responses to cell perturbations is limited by the vast diversity of messenger RNA (mRNA) and protein half-lives. At early time points, detectable changes in mRNA abundance are inevitably biased toward short-lived transcripts, while analyses at later time points do not allow for distinguishing direct from secondary effects. We have developed a simple, scalable method for enabling direct detection of 4-thiouridine (4sU)-labeled transcripts within the total RNA pool, which can be combined with standard RNA sequencing methods for investigating dynamic changes in gene expression. We have named this technique “thiol (SH)-Linked Alkylation for the Metabolic sequencing of RNA” (SLAMseq). One key application of SLAMseq is to directly quantify specific or global changes in mRNA output following pharmacological or chemical–genetic gene perturbation, and to thereby define primary transcriptional functions of genes and drugs.
During the webinar, the speakers will:
- Explain the basic principles and experimental implementation of SLAMseq
- Outline the method’s utility for probing direct transcriptional targets of regulatory genes as well as primary transcriptional responses to drug treatment
- Discuss future applications
- Answer questions from the online audience during the live broadcast!
This webinar will last for approximately 60 minutes.