Three high-performance, open-source approaches—NanoJ-SRRF, NanoJ-SQUIRREL, and NanoJ-Fluidics—have recently been developed to enable and enhance optical superresolution microscopy in most modern microscopes. NanoJ-superresolution radical fluctuations (SRRF) is a new superresolution method enabling live-cell nanoscopy with illumination intensities orders of magnitude lower than techniques such as single-molecule localization microscopy (SMLM) or stimulated emission depletion (STED) microscopy can deliver. SRRF’s low phototoxicity allows unprecedented imaging for long acquisition times at resolutions equivalent to or better than those possible with structured illumination microscopy (SIM). NanoJ-SQUIRREL (superresolution quantitative image rating and reporting of error locations), an analytical approach that provides quantitative assessment of superresolution image quality, can guide researchers in optimizing imaging parameters. By comparing diffraction-limited images and superresolution equivalents of the same acquisition volume, this method generates a quality score and quantitative map of superresolution defects. NanoJ-Fluidics is a novel fluidics technique for automating complex sequences of treatment, labeling, and imaging of live and fixed cells with high reproducibility.
During the webinar, speakers will:
- Showcase how SRRF benefits from spinning-disk acquisition and performs with EM-CCDs and sCMOS cameras
- Demonstrate how NanoJ-Fluidics can be used to examine actin dynamics, nanoscale cell topography, and the role of adhesion contacts in mitosis
- Answer your questions during the live broadcast!
This webinar will last for approximately 60 minutes