The vast majority of clinical samples are still archived using a decades-old technique of fixation with formaldehyde and embedding in paraffin wax to generate so-called formalin-fixed paraffin-embedded (FFPE) tissue blocks. This process of fixation and embedding, as well as long-term storage, can result in severe degradation of nucleic acids in the tissue. The fixation process itself causes cross-linkage between nucleic acids and proteins, as well as covalent modification of nucleic acids by monomethylol (-CH2OH) addition to the bases. Millions of FFPE blocks form the foundation of many tissue archives worldwide, and are essential to clinical research for exploring disease pathways and developing novel therapies. The inherent challenges of obtaining useable nucleic acids from difficult sample types such as these can be overcome by using the latest technologies and procedures to provide meaningful results throughout the next-generation sequencing workflow, from initial extraction of DNA/RNA to sample quantification, sample quality control, and library preparation.
During this webinar, our experts will:
- Describe the types of degradation challenges found with FFPE samples
- Introduce the range of technologies available to mitigate FFPE nucleic-acid damage
- Present recent data using difficult and degraded tissue samples in cancer research
- Answer your questions live during the broadcast!
This webinar will last for approximately 60 minutes