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Immune profiling of single cells: A new milestone in multiomic biomarker analysis

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Immune profiling of single cells: A new milestone in multiomic biomarker analysis

30 January 2019

12:00 p.m. ET

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Speaker

The ability to simultaneously interrogate protein and mRNA information in thousands of single cells is a recent development allowing greater insight into the biology of different disease states. Combined measurement of specific proteins and gene transcripts in individual cells is critical for understanding the role of cellular diversity in development, health, and disease, leading to more accurate insights into complex biological systems. These powerful tools allow researchers to gain a more comprehensive understanding of the intricacies of immune-related diseases, thus providing opportunities for more personalized treatments.

During this webinar, our expert speaker will describe how the power of multiomic single-cell analysis was applied in recent asthma research, in which thousands of T cells were captured using oligo-conjugated cell-surface antibodies and interrogated using targeted sequencing to identify immune-cell biomarkers. A digital gene-expression profile of thousands of individual cells was generated in a single experiment, without the need for robotics or automation, enabling identification of distinct cell-surface proteins and hundreds of gene-expression biomarkers.

In the webinar, viewers will:

  • Learn about single-cell analysis methods based on RNA sequencing (RNAseq) and antibody oligonucleotide conjugates (AbSeq) technologies
  • Understand the advantages of combining data from transcriptomics and proteomics analysis
  • Gain insight into how single-cell multiomic methods can be applied to identify biomarkers and cell subsets using asthma as a model
  • Be able to ask questions during the live broadcast.

This webinar will last for approximately 60 minutes.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Speaker bios

Swati Acharya, Ph.D.

Stanford University
Palo Alto, CA

Dr. Acharya obtained her Ph.D. in cancer genetics and immunology from Tufts University School of Medicine. During her postdoctoral fellowships in immunology–rheumatology at Stanford University School of Medicine, she worked with Garry Fathman, using mouse models to understand the role of GRAIL (gene related to anergy in lymphocytes), an Akt interactor, and other markers in regulatory T-cell development; and with David B. Lewis, using clinical samples to investigate the mechanism of pseudokinase PTK7 (protein tyrosine kinase 7) in primary human thymocytes, neonatal and adult T-cell development, and cancer. In 2017, Dr. Acharya joined the Nadeau Laboratory at Stanford University, where she applies her knowledge of immunology, genetics, and mechanistic pathways of immune modulation in T-cell development to identify molecular mechanisms and biomarkers of impaired regulatory T (Treg) cells and Type 2 T helper (Th2) cells in asthma and allergy. She investigates clinical samples from twin cohorts using cutting-edge 'omics, such as ATACseq (Assay for Transposase-Accessible Chromatin using sequencing), single-cell transcriptomics, and CyTOF-based proteomics, together with targeted single-cell and plasma mass spectrometry and other novel methods.

Jackie Oberst, Ph.D.

Science/AAAS
Washington, D.C.

Dr. Oberst did her undergraduate training at the University of Maryland, College Park, and her Ph.D. in Tumor Biology at Georgetown University, Washington D.C. She combined her interests in science and writing by pursuing an M.A. in Journalism from the Philip Merrill College of Journalism at the University of Maryland, College Park. Dr. Oberst joined Science/AAAS in 2016 as the Assistant Editor for Custom Publishing. Before then she worked at Nature magazine, the Howard Hughes Medical Institute, The Endocrine Society, and the National Institutes of Mental Health.

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