Sequencing of RNA (RNASeq) can provide a trove of useful information for quantification and characterization of the transcriptome. During bulk sequencing, however, spatial and cellular heterogeneity information is lost. Enter single-cell–level analysis, which can provide unprecedented detail on cellular diversity in development, health, and disease. This emerging area of spatial transcriptomics aims to map a single-cell–level molecular atlas of human tissues in situ. However, mapping entire tissue areas at single-cell quantitative resolution presents significant challenges for imaging and data analysis. In this webinar, efforts to develop large-scale in situ transcriptomics will be described, using the mouse brain as a model system for mapping the molecular diversity of neuronal and glial cell types. The experimental workflow includes automated tissue processing, imaging and analysis for large-scale multiplex mapping, and quantification of gene expression across brain regions in situ at single-cell resolution. The technology that makes this high-resolution mapping possible will be described and compared with other current methods.
During the webinar, the speakers will:
- Describe the power and usefulness of in situ transcriptomics
- Discuss the application of this technology to large-scale tissue mapping in the mouse brain
- Explain how in situ transcriptomics (using RNAScope technology) can complement RNASeq and single-cell RNASeq
- Answer audience questions during the live broadcast!
To learn more about products or technologies related to this webinar, go to http://rna.acdbio.com/transcriptomics.
This webinar will last for approximately 60 minutes.