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Generating the best superresolution microscopy data: Finding the right tool for the right job

This webinar is brought to you by the Science/AAAS Custom Publishing Office

Generating the best superresolution microscopy data: Finding the right tool for the right job

Recorded 29 July 2015

Speakers

Superresolution microscopy has recently been making headlines again, not least because it was the breakthrough that spurred Eric Betzig, Stefan Hell, and William Moerner to be awarded the Nobel Prize for Chemistry in 2014. The number of superresolution technologies keeps increasing, along with an alphabet soup of acronyms that includes SIM, SPIM, STED, PALM, and STORM. It is difficult enough for the microscopy experts to keep up, but even more challenging for the average biologist, particularly when they are looking for more from their data than a standard or confocal microscope can offer. When faced with the array of possible techniques, how does one decide which technique is going to get the results needed for that next major publication? In this webinar we will discuss the newest developments in superresolution microscopy and provide practical advice on obtaining the best imaging results. Our panelists, including 2014 Nobel Prize winner Eric Betzig, will help you find the right tool to answer your biological question. You will also hear case studies about how superresolution microscopy data has made the difference between acceptance and rejection of key papers by scientific journals. During this webinar you will learn:

  • the array of superresolution techniques available, and which is best for you
  • which techniques are optimal for live cell imaging
  • which techniques give the best resolution
  • which techniques are best for deep tissue imaging
  • for which techniques you can use your existing fluorophores and buffers, and for which you will need to reoptimize your sample preparation.

 You will also be able to submit questions directly to the panelists during the live event.

The webinar will last approximately 60 minutes and will be followed by a live webchat (see below).

To learn more about products or technologies related to this webinar, go to: www.gelifesciences.com/deltavision

Post-webinar webchat

Live Blog Webchat: Generating the best superresolution microscopy data
 

 

Speaker bios

Eric Betzig, Ph.D.

HHMI Janelia Research Campus
Ashburn, VA

Dr. Betzig obtained a B.S. in physics from Caltech and a Ph.D. in applied physics from Cornell University. In 1988, he became a principal investigator at AT&T Bell Laboratories where he extended his thesis work on near-field optical microscopy, the first method to break the diffraction barrier. By 1993, he held a world record for data storage density, and recorded the first superresolution fluorescence images of cells as well as the first single molecule images at ambient temperature. Frustrated with technical limitations and declining standards as more jumped into the field, he quit science and by 1996 was working for his father's machine tool company. Commercial failure of the technologies he developed there left him unemployed in 2003 and looking for new directions. This search eventually culminated in his co-invention of the superresolution technique PALM (photo-activated localization microscopy) with his best friend, Bell Labs colleague Harald Hess. For this work, he was co-recipient of the 2014 Nobel Prize in Chemistry. Since 2005, he has been a group leader at the Janelia Research Campus, developing new optical imaging technologies for biology.

Raman Das, Ph.D.

University of Dundee
Dundee, UK

Dr. Das completed undergraduate training in genetics as well as his Ph.D. in molecular and developmental biology at the University of Sheffield in the United Kingdom. He currently holds a research associate position under Kate Storey in the College of Life Sciences, University of Dundee in the United Kingdom. His work involves high-resolution imaging of chick embryonic tissue slices to study the cell-biological mechanisms driving neurogenesis in the spinal cord, for which Dr. Das played an instrumental role in pioneering new imaging technology. The work, recently published in Science, led to the discovery of a new form of cell subdivision. In 2014, Dr. Das was awarded the Tim Hunt Prize for Cell Biology from the College of Life Sciences at the University of Dundee.

Justin Taraska, Ph.D.

National Institutes of Health
Bethesda, MD

Dr. Taraska received his B.A. in biology from Reed College in 1999 and earned his Ph.D. in cell biology from Oregon Health and Science University in 2004 in the laboratory of Wolfhard Almers. While working on his Ph.D., Dr. Taraska investigated the processes of triggered exocytosis and endocytosis in neuroendocrine cells with high-resolution microscopy methods. He conducted his postdoctoral research in the laboratory of William Zagotta at the University of Washington where he received a Jane Coffin Child Memorial Fellowship. During his postdoc, Dr. Taraska developed and used novel fluorescence methods to study the structure of ion channels in biological membranes. In 2010, Dr. Taraska became an Investigator at the National Heart Lung and Blood Institute, National Institutes of Health. Dr. Taraska is a 2012 Presidential Early Career Award for Scientists and Engineers recipient. He is also the co-director of the analytical and quantitative light microscopy course at the Marine Biological Lab in Woods Hole, Massachusetts. Dr. Taraska’s lab studies the structural cell biology of exocytosis and endocytosis with advanced imaging methods including live cell microscopy, superresolution fluorescence, and electron microscopy. 

Sean Sanders, Ph.D.

Science/AAAS
Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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