In vitro high throughput screening in drug discovery has typically been accomplished using reporter gene assays, which offer a versatile, cost-effective, and technically simple way to screen in high throughput, and are amenable to miniaturization. However, these assays are unsuitable for screening primary cells and cannot assess impact on endogenous gene expression. Real-time (quantitative) PCR (qPCR) can overcome these drawbacks and has widespread and proven use in gene expression analysis at both low and medium throughput. Until now, the application of qPCR in high throughput screening (HTS) has been limited by an often laborious multistep process to generate suitable template, and high reagent cost due to large reaction volumes. Recent advances in streamlining the qPCR workflow, liquid handling, and instrumentation have enabled scientists to generate sensitive and cost-effective high throughput qPCR data in drug discovery processes.
During the webinar the expert panel will:
- Describe the advances in technology that have enabled the application of qPCR in HTS
- Discuss the pros and cons of qPCR vs. traditional reporter gene assays in HTS
- Outline the necessary steps required for fully automated implementation of qPCR technology in HTS
- Answer your questions live during the broadcast.
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