Webinar Technology

The emergence of structured illumination microscopy: From home-built to commercial solutions

This webinar is brought to you by the Science/AAAS Custom Publishing Office

The emergence of structured illumination microscopy: From home-built to commercial solutions

Recorded 30 November 2016

Speakers

The diffraction limit for light microscopy (approximately half the wavelength of light used to image a sample) was for many years a barrier to truly high-resolution microscopy. Superresolution microscopy came about through the recognition that clever hardware design and digital image processing could break through the diffraction limit, enabling scientists to image down to the molecular level. Of all the superresolution techniques, structured illumination microscopy (SIM) is probably the most relevant for live samples, primarily because it is the most versatile and suitable for fairly long exposures and dynamic cell imaging. Stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM), as well as techniques derived from them, provide higher-resolution images but tend to require considerably more laser power than SIM—making phototoxicity a significant problem. This webinar will provide a summary of superresolution microscopy, with a particular focus on SIM, and will highlight the important factors to consider when undertaking microscopy experiments, including hardware, software, and samples.

During the webinar, viewers will:

  • Learn the basics of superresolution microscopy and how to achieve the best images using different techniques 
  • Be introduced to light-sheet microscopy as a means to achieve high-resolution 3D images while reducing phototoxicity 
  • Gain insight into the impact of hardware such as camera type on imaging results. 

This webinar will last for approximately 60 minutes

Speaker bios

Bi-Chang Chen, Ph.D.

Academia Sinica
Taipei, Taiwan

Dr. Chen received his B.S. and M.S. in chemistry from National Taiwan University in Taipei, Taiwan. For his M.S., he worked on mesoporous materials synthesis under the supervision of Professor Chung-Yuan Mou. In 2006 he moved to the United States to complete his Ph.D. in chemistry at the University of Texas at Austin, where he studied coherent anti-Stokes Raman scattering (CARS) imaging in Dr. Sang-Hyun Lim’s group. He joined Dr. Eric Betzig’s group in 2011, to continue his postdoctoral work developing cutting-edge imaging tools such as lattice light-sheet microscopy for 3D live fluorescence imaging. In 2014 he joined Academia Sinica in Taiwan as an assistant research fellow in the Research Center for Applied Sciences. Dr. Chen is interested in developing fast, low-phototoxicity, multicolor, and 3D detection for fluorescent living specimens using subcellular resolution imaging tools.

George Komis, Ph.D.

Palacký University
Olomouc, Czech Republic

Dr. Komis is assistant professor at the Center of the Region Haná for Biotechnological and Agricultural Research (CRH) of Palacký University in Olomouc, Czech Republic. He received his undergraduate and Ph.D. degrees from the University of Athens, Greece (Laboratory of Plant Cell Biology, Department of Biology). His Ph.D. thesis addressed how the microtubule and actin cytoskeleton reorganizes during the hyperosmotic stress response of plant cells by using cell volume regulatory mechanisms. He continued these studies during his postdoctoral training at the same university, while working as a DAAD (German Academic Exchange Service) fellow in Biocenter Klein Flottbek (University of Hamburg, Germany), and as an Alexander von Humboldt fellow in the laboratory of Professor Jozef Šamaj (Institute of Cellular and Molecular Botany, University of Bonn, Germany). In the CRH, Dr. Komis works on plant cytoskeletal remodeling and its integration with mitogen-activated protein kinase (MAPK) signaling. He develops imaging procedures over a wide range of spatiotemporal resolutions using confocal laser scanning, spinning disc, structured illumination, photoactivation localization, and lightsheet microscopies, in order to elucidate the dynamics of different plant microtubule arrays in dividing, differentiating, or environmentally challenged plant cells.

Christelle Rosazza, Ph.D.

Andor Technology
Belfast, Northern Ireland

Dr. Rosazza works at Andor Technology as a global application specialist in life science. Her role brings her in touch with the latest developments and innovations in both camera technology and microscopy applications. Previous to this position, Dr. Rosazza comanaged a microscopy facility in the Department of Systems and Synthetic Microbiology at the Max Planck Institute for Terrestrial Microbiology in Marburg, Germany. She worked with a variety of microscopy systems including automated wide-field fluorescent microscopy, confocal microscopy, and TIRF/FRAP/FRET systems. Her interest in microscopy stemmed from her Ph.D. obtained in the Department of Chemistry at the University of Konstanz in Germany, where she employed techniques such as single-particle tracking in mammalian cells.

Sean Sanders, Ph.D.

Science/AAAS
Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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