The diffraction limit for light microscopy (approximately half the wavelength of light used to image a sample) was for many years a barrier to truly high-resolution microscopy. Superresolution microscopy came about through the recognition that clever hardware design and digital image processing could break through the diffraction limit, enabling scientists to image down to the molecular level. Of all the superresolution techniques, structured illumination microscopy (SIM) is probably the most relevant for live samples, primarily because it is the most versatile and suitable for fairly long exposures and dynamic cell imaging. Stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM), as well as techniques derived from them, provide higher-resolution images but tend to require considerably more laser power than SIM—making phototoxicity a significant problem. This webinar will provide a summary of superresolution microscopy, with a particular focus on SIM, and will highlight the important factors to consider when undertaking microscopy experiments, including hardware, software, and samples.
During the webinar, viewers will:
- Learn the basics of superresolution microscopy and how to achieve the best images using different techniques
- Be introduced to light-sheet microscopy as a means to achieve high-resolution 3D images while reducing phototoxicity
- Gain insight into the impact of hardware such as camera type on imaging results.
This webinar will last for approximately 60 minutes