Webinar Technology

CRISPR unleashed: New tools and applications in live-cell imaging

This webinar is brought to you by the Science/AAAS Custom Publishing Office

CRISPR unleashed: New tools and applications in live-cell imaging

Recorded 29 November 2017


The CRISPR/Cas9 gene editing system has been a boon for researchers, enabling them to manipulate a broad range of genomes quickly and accurately. This novel, versatile tool has been used with great precision for DNA editing as well as a multitude of other applications. Recent enhancements have expanded its abilities; one example is a new, hyperaccurate Cas9 variant that demonstrates high specificity across the genome without compromising on-target activity in human cells. Another is a modification of Cas9 called CRISPRainbow, a system that can more easily label multiple genomic loci in living cells, allowing researchers to better study chromatin dynamics. The unprecedented flexibility of the CRISPR toolset makes it possible to capture imaging phenomena happening at very small scales of space and time. An essential piece of this technology is a camera that captures the fine structure necessary to see the modified DNA (and distinguish it from the background). It must be capable of functioning in challenging environments in living cells, which require cutting-edge light manipulation and detection technologies optimized for light-starved conditions. During this webinar, our experts will examine some of the latest enhancements to the CRISPR/Cas9 system and explain how they are being applied in the lab today.

During the webinar, viewers will:

  • Be introduced to the CRISPR/Cas9 gene editing technology and how it is currently being applied at the bench
  • Learn about the latest improvements to CRISPR/Cas9 that go beyond DNA manipulation
  • Hear about the latest camera-based imaging technologies for use in conjunction with new CRIPSR modalities in low-light, live-cell environments
  • Have the opportunity to ask questions live during the broadcast!

This Webinar will last for approximately 60 minutes.

Speaker bios

David Grunwald, Ph.D.

University of Massachusetts School of Medicine
Worcester, MA

Dr. Grunwald received his Ph.D. in biophysics at the Ludwig-Maximilian University of Munich, in Germany, in 2006. For his postdoctoral work, he joined the Robert H. Singer Lab at Albert Einstein College of Medicine of Yeshiva University in New York, leaving in 2010 to move to the Kavli Institute for Nanoscience Delft, at Delft University of Technology in the Netherlands, finally transitioning to his current position at the University of Massachusetts Medical School in 2012. He was the first to measure the transition of a messenger RNA through the nuclear pore complex, determining that it was orders of magnitude faster than expected and that the rate-limiting steps in this process are at the nuclear and cytoplasmic surfaces.

Ahmet Yildiz, Ph.D.

University of California, Berkeley
Berkeley, CA

Dr. Yildiz obtained his Ph.D. in biophysics under Dr. Paul Selvin at the University of Illinois Urbana–Champaign in 2004. After completing his postdoctoral work with Dr. Ron Vale at the University of California, San Francisco, he joined the Physics Department at the University of California, Berkeley in 2008. His research focuses on the development of high-precision, single-molecule methods to study the mechanism of action of macromolecular machines, such as dynein, telomerase, and Cas9. His work has been recognized by the GE & Science Prize for Young Life Scientists, the Michael and Kate Bárány Award, the American Society for Cell Biology-Gibco Emerging Leader Prize, and the Vilcek Prize for Creative Promise.

Marcin Barszczewski, Ph.D.

Andor Technology
Belfast, Northern Ireland

Dr. Barszczewski is a Belfast-based research applications scientist and training specialist at Andor Technology. He has 15 years’ expertise in a wide range of imaging applications for CCD, EMCCD, and CMOS cameras as well as diverse microscopy and image-data visualization systems. He obtained his Ph.D. in neuroscience from the Max-Planck Institute for Biophysical Chemistry and the University of Goettingen, Germany in the lab of Reinhard Jahn. He has held various technical roles at Andor, now an Oxford Instruments company, advising customers in areas of astronomy and low-light biomedical imaging as well as physical imaging, including X-ray and quantum imaging, and advanced image-data visualization and analysis. He has been involved in the creation of application notes and technical white papers on established and emerging imaging applications, trends, and technologies. In addition, he continues to be actively involved in supporting pan-European and global research projects within the European Union’s Framework Programmes. Dr. Barszczewski’s specialties include biomedical, low-light and single-molecule imaging, molecular neuroscience, and scientific writing.

Sean Sanders, Ph.D.

Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Senior Editor for Custom Publishing for the journal Science and Program Director for Outreach.

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