Antibodies are invaluable tools in the life sciences. There are several challenges that must be overcome to validate the specificity and reproducibility of antibody reagents, as emphasized by many publications, including a recent proposal from the International Working Group for Antibody Validation (IWGAV). There are many examples of antibodies with off-target binding leading to erroneous conclusions, some of which will be discussed during this webcast, demonstrating the importance of application-specific validation. Five alternative strategies for enhanced validation of antibodies will be described based on genetic validation, recombinant expression, independent antibodies, orthogonal validation, and capture mass spectrometry validation. Approaches based on these five strategies have been used for streamlined, enhanced validation of more than 10,000 antibodies from more than 30 providers, targeting over 7,000 human proteins as part of the Human Protein Atlas program. The results show a path forward for validating antibodies in an application-specific manner suitable for both providers and users
During the webinar, viewers will learn about:
- The generation and validation of data used in the Human Protein Atlas
- Problems related to the cross-reactivity of antibodies and the challenges they create for the interpretation of results
- Enhanced validation of antibodies using five application-specific strategies.
Speakers will answer your questions during the live broadcast!
This webinar will last for approximately 60 minutes.