Cultrex Organoid Progenitor Cells, derived from normal, healthy mouse small intestine tissue, are now available and suitable for gene editing. These cells can be expanded using reduced growth factor (RGF) BME-R1, and may be induced to express tissue-specific markers under differentiating conditions. Cultrex cells will be of particular interest to the large number of leading research groups worldwide that specialize in drug screening applications and use BME 2 organoid matrices. These cells are also ideally suited for use in common gene-editing techniques such as CRISPR/Cas9, for developing models for infectious disease and cancer, and for studying the normal intestine. Organoid 3D cultures are extracted directly from living tissues similar to primary cultures. Instead of using an artificial, tissue culture–treated plastic environment, stem cell populations are maintained using an extracellular matrix environment under nondifferentiating conditions. When subjected to differentiating conditions, these organoids exhibit expression of tissue-specific genes and differentiation of stem cells into tissue-specific architecture.