The CellASIC ONIX2 Microfluidic System converts laboratory microscopes into powerful tools for live cell imaging, to perform in-depth analysis of cellular mechanisms and behavior. It allows precise control and manipulation of cell culture environments, and provides constant, stable imaging conditions while preserving the health of cells. The all-in-one platform consists of a controller that maintains environmental conditions, application-specific microfluidic plates, and intuitive software. With the ONIX2, researchers can monitor cellular responses to environmental changes such as media, stimulants, temperature, and gas, and track individual cells over time. The system provides a new level of reliability to researchers studying long-term growth, movement, signaling, and cellular interactions and responses to environmental stimuli, including hypoxia and cancer cell behavior. Its high-resolution capabilities are ideal for laboratories interested in producing time-lapse cell culture videos or developing cell-based assays and optimizing parameters for short- or long-term cultures.