ZymoBIOMICS Microbial Community Standards contain two well-defined and characterized standards to validate each step of your workflow. The first is a mock microbial community comprising eight bacteria and two fungi, which is used to validate a DNA isolation protocol. The second is a DNA standard from these same organisms for validating your sequencing and data analysis. The microbial community standards are accurately characterized, with a wide guanine–cytosine (GC) range (15%–85%), and contain negligible impurities (<0.01%). The DNA Standard is ideal for assessing biases and errors in library preparation, sequencing, and bioinformatics analyses. It serves perfectly as a microbial standard for benchmarking the performance of microbiomics or metagenomics analyses and as a control in interlab studies. This standard is also ideal for helping users construct and optimize workflows (e.g., controlling PCR chimera rate and noise in the library preparation of 16S ribosomal RNA gene–targeted sequencing, and assessing GC bias in coverage of shotgun metagenomic sequencing).