The IncuCyte ZOOM System enables observation and quantification of cell behavior over time by automatically gathering and analyzing images around the clock within a standard incubator. Whether it is for simple cell-proliferation assays or more advanced stem cell differentiation biology, our live cell, nonperturbing imaging approach yields kinetic data and insight far beyond that achievable with conventional endpoint or nonimage based approaches. The system consists of a microscope gantry that resides in the cell incubator, and a networked external-controller hard drive that gathers and processes image data. Different microscope objectives (4x, 10x, 20x) can be housed within each system and readily interchanged by the user. Each IncuCyte ZOOM houses multiple T-flasks or microtiter plates (up to six) and can acquire >2,000 images per hour. The platform supports high-definition phase contrast, as well as two-color (green and red) fluorescence automated-imaging modes. The IncuCyte ZOOM is controlled from the office or home by intuitive, easy-to-use desktop software.