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Antibody staining of a Drosophila wing imaginal disc (500 micrometers long) for the activated form of MAP kinase (dp-ERK) (green) and Argos (red). With antibody to dp-ERK, the activated state of signaling pathways triggered by receptor tyrosine kinases can be followed in situ, revealing the time and amount of activation, the extent of ligand diffusion, and the effects of inhibitory ligands. The regions of maximal epidermal growth factor receptor activation correspond to the future wing veins. See page 1103. [Image: L. Gabay, R. Seger, B-Z. Shilo]

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Science. ISSN 0036-8075 (print), 1095-9203 (online)