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Single-molecule fluorescence spectroscopy has shed light on many biological processes, from protein folding to enzyme dynamics. However, to study weakly interacting molecules, the diffraction limit of light must be overcome. In their Perspective, Laurence and Weiss review recent "superresolution" methods that achieve smaller detection volumes, a requirement for studying weak interactions. They highlight the method of Leveneet al., who achieve parallel detection of many single molecules in extremely small (zeptoliter) detection volumes.
T. A. Laurence is in the Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA. E-mail: laurence{at}chem.ucla.edu S. Weiss is in the Department of Chemistry and Biochemistry, the Department of Physiology, David Geffen School of Medicine at UCLA, and the California Nanosystems Institute, University of California, Los Angeles, CA 90095, USA. E-mail: sweiss{at}chem.ucla.edu
